Overview

  • Product nameAnti-LHX3 antibody
    See all LHX3 primary antibodies
  • Description
    Rabbit polyclonal to LHX3
  • SpecificityAb14555 recognizes LHX3 and does not appear to crossreact with gsh4 on tissue sections.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human, Fish
  • Immunogen

    C-terminal portion of mouse LHX3 protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab14555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
IHC-Fr
WB
IP
  • Application notesICC: 1/200 - 1/500.
    IF: Use at an assay dependent dilution. Use a secondary antibody conjugated to biotin and then streptavidin-conjugated to fluorochrome.
    IHC (whole mounts): 1/4000 - 1/8000. Use in conjunction with MEMFA as fixative.
    IHC-P: 1/250 - 1/1000.
    IHC-Fr: 1/3000 - 1/6000.
    IP: 1/200.
    WB: 1/2500 - 1/5000. Predicted molecular weight: 44 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with Pit-1.
    • Involvement in diseaseDefects in LHX3 are the cause of pituitary hormone deficiency combined type 3 (CPHD3) [MIM:221750]; also known as combined pituitary hormone deficiency with rigid cervical spine or sensorineural deafness with pituitary dwarfism. CPHD is characterized by a complete deficit in all but one (adrenocorticotropin) anterior pituitary hormone and a rigid cervical spine leading to limited head rotation.
    • Sequence similaritiesContains 1 homeobox DNA-binding domain.
      Contains 2 LIM zinc-binding domains.
    • DomainThe LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CPHD 3 antibody
      • CPHD3 antibody
      • DKFZp762A2013 antibody
      • LHX 3 antibody
      • LHX3 antibody
      • LHX3_HUMAN antibody
      • LIM 3 antibody
      • LIM homeobox 3 antibody
      • LIM homeobox gene 3 antibody
      • LIM homeobox protein 3 antibody
      • LIM/homeobox protein Lhx3 antibody
      • LIM/homeodomain protein LHX3 antibody
      • Lim3 antibody
      • M2 LHX3 antibody
      • mLim-3 antibody
      • mLIM3 antibody
      • P LIM antibody
      see all

    References for Anti-LHX3 antibody (ab14555)

    This product has been referenced in:
    • Soundararajan P  et al. Guidance of postural motoneurons requires MAPK/ERK signaling downstream of fibroblast growth factor receptor 1. J Neurosci 30:6595-606 (2010). IHC-Fr ; Chicken . Read more (PubMed: 20463222) »
    • Ji SJ  et al. Nolz1 is induced by retinoid signals and controls motoneuron subtype identity through distinct repressor activities. Development 136:231-40 (2009). Read more (PubMed: 19056829) »

    See all 3 Publications for this product

    Product Wall

    Thank you for your enquiry. I regret to inform you that there are no details available regarding the characterization of ab14555 in Western blotting. Often it is possible to make suggestions that help resolve problems. We will happily offer technic...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"