Overview

  • Product nameAnti-LHX3 antibody [EPR3344(2)]See all LHX3 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR3344(2)] to LHX3
  • Tested applicationsWB, IP, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues from Human LHX3.

  • Positive control
    • 293T and Jurkat cell lysates, Human brain and fetal lung tissue lysates; permeabilized Jurkat cells.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab124697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionActs as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with Pit-1.
    • Involvement in diseaseDefects in LHX3 are the cause of pituitary hormone deficiency combined type 3 (CPHD3) [MIM:221750]; also known as combined pituitary hormone deficiency with rigid cervical spine or sensorineural deafness with pituitary dwarfism. CPHD is characterized by a complete deficit in all but one (adrenocorticotropin) anterior pituitary hormone and a rigid cervical spine leading to limited head rotation.
    • Sequence similaritiesContains 1 homeobox DNA-binding domain.
      Contains 2 LIM zinc-binding domains.
    • DomainThe LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.
    • Cellular localizationNucleus.
    • Target information above from: UniProt accession Q9UBR4 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • CPHD 3 antibody
      • CPHD3 antibody
      • DKFZp762A2013 antibody
      • LHX 3 antibody
      • LHX3 antibody
      • LHX3_HUMAN antibody
      • LIM 3 antibody
      • LIM homeobox 3 antibody
      • LIM homeobox gene 3 antibody
      • LIM homeobox protein 3 antibody
      • LIM/homeobox protein Lhx3 antibody
      • LIM/homeodomain protein LHX3 antibody
      • Lim3 antibody
      • M2 LHX3 antibody
      • mLim-3 antibody
      • mLIM3 antibody
      • P LIM antibody
      see all

    Anti-LHX3 antibody [EPR3344(2)] images

    • All lanes : Anti-LHX3 antibody [EPR3344(2)] (ab124697) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Human brain tissue lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Human fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 43 kDa
    • ab124697 at 1/10 dilution, staining LHX3 in permeabilized Jurkat cells by flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-LHX3 antibody [EPR3344(2)] (ab124697)

    ab124697 has not yet been referenced specifically in any publications.

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