Overview

  • Product name
  • Description
    Rabbit polyclonal to Lhx4
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 287-336 (GSFSMDGTGQ SYQDLRDGSP YGIPQSPSSI SSLPSHAPLL NGLDYTVDSN) of Human Lhx4 (NP_203129).

  • Positive control
    • 721_B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    May play a critical role in the development of respiratory control mechanisms and in the normal growth and maturation of the lung.
  • Involvement in disease
    Defects in LHX4 are the cause of pituitary hormone deficiency combined type 4 (CPHD4) [MIM:262700]; also known as short stature pituitary and cerebellar defects and small sella turcica. The disorder is characterized by short stature, pituitary and cerebellar defects, and small transverse depression crossing the midline on the superior surface of the body of the sphenoid bone which houses the pituitary gland.
    Note=A chromosomal aberration involving LHX4 may be a cause of acute lymphoblastic leukemia. Translocation t(1;14)(q25;q32) with IGHG1.
  • Sequence similarities
    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CPHD4 antibody
    • Gsh 4 antibody
    • Gsh4 antibody
    • Lhx4 antibody
    • LHX4_HUMAN antibody
    • LIM Homeobox 4 antibody
    • LIM Homeobox gene 4 antibody
    • LIM homeobox protein 4 antibody
    • LIM/homeobox protein Lhx4 antibody
    see all

Images

  • Anti-Lhx4 antibody (ab84519) at 1 µg/ml (5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
    Observed band size : 41 kDa

References

ab84519 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse embryonic spinal cord (cryosections))
Permeabilization
Yes - 0.1%Triton X-100
Specification
Mouse embryonic spinal cord (cryosections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Dr. Frederic Clotman

Verified customer

Submitted Nov 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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