Overview

  • Product nameAnti-LHX5 antibody
    See all LHX5 primary antibodies
  • Description
    Mouse monoclonal to LHX5
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish
  • Immunogen

    Recombinant fragment: CTDRSLSPDL QDALQDDPKE TDNSTSSDKE TANNENEEQN SGTKRRGPRT TIKAKQLETL KAAFAATPKP TRHIREQLAQ ETGLNMRVIQ VWFQNRRSKE, corresponding to amino acids 136-235 of Human LHX5 (NP_071758) with a proprietary tag.

  • Positive control
    • Partial recombinant tagged LHX5 protein. 293, MES-SA/DX5, Y-79, and Jurkat cell lysates. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 44 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged LHX5 is approximately 3ng/ml when used as a capture antibody.
ICC/IF Use a concentration of 10 µg/ml.

Target

Anti-LHX5 antibody images

  • Anti-LHX5 antibody (ab88185) at 1 µg/ml + Partial recombinant tagged LHX5 (Immunogen) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 44 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-LHX5 antibody (ab88185) at 1 µg/ml + 293 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
  • Anti-LHX5 antibody (ab88185) at 1 µg/ml + MES-SA/Dx5 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.
  • Anti-LHX5 antibody (ab88185) at 1 µg/ml + Y-79 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 44 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
  • Anti-LHX5 antibody (ab88185) at 1 µg/ml + Jurkat cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
  • ab88185, at 10 µg/ml, staining LHX5 in permeabilised, paraformaldehyde fixed HeLa cells by immunofluorescence.

References for Anti-LHX5 antibody (ab88185)

ab88185 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"