Overview

  • Product nameAnti-LHX6 antibody
    See all LHX6 primary antibodies
  • Description
    Rabbit polyclonal to LHX6
  • Specificityab22885 recognises LHX6
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow, DogDoes not react with: Mouse
  • Immunogen

    Synthetic peptide: RRVIQVWFQN CRARHKKHTP QHPVPPSGAP PSRLPSALSD DIHYTPFSSP, corresponding to a region within C terminal amino acids 289-338 of Human LHX6

  • Positive control
    • HepG2 cell lysate and human liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab22885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionProbable transcription factor required for the expression of a subset of genes involved in interneurons migration and development. Functions in the specification of cortical interneuron subtypes and in the migration of GABAergic interneuron precursors from the subpallium to the cerebral cortex.
  • Sequence similaritiesContains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • LHX 6 antibody
    • LHX 6.1 antibody
    • LHX6 antibody
    • LHX6.1 antibody
    • LHX6_HUMAN antibody
    • LIM homeobox 6 antibody
    • LIM homeobox protein 6 antibody
    • LIM homeodomain protein 6.1 antibody
    • LIM/homeobox protein Lhx6 antibody
    • LIM/homeobox protein Lhx6.1 antibody
    • MGC119542 antibody
    • MGC119544 antibody
    • MGC119545 antibody
    • OTTHUMP00000064113 antibody
    • OTTHUMP00000064114 antibody
    see all

Anti-LHX6 antibody images

  • Immunohistochemical analysis of LHX6 expression in paraffin embedded human liver tissue. ab22885 was used at 4 ug/ml. Arrows indicate positively labelled hepatocytes.
  • Lane 1 : Anti-LHX6 antibody (ab22885) at 5 µg/ml
    Lane 2 : Anti-LHX6 antibody (ab22885) at 2.5 µg/ml
    Lane 3 : Anti-LHX6 antibody (ab22885) at 1.25 µg/ml

    Lane 1 : Cell lysate prepared from human HepG2 cells
    Lane 2 : Cell lysate prepared from human HepG2 cells
    Lane 3 : Cell lysate prepared from human HepG2 cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-LHX6 antibody (ab22885)

This product has been referenced in:
  • García-Moreno F  et al. A neuronal migratory pathway crossing from diencephalon to telencephalon populates amygdala nuclei. Nat Neurosci 13:680-9 (2010). IHC-P ; Mouse . Read more (PubMed: 20495559) »
  • Jakovcevski I  et al. Multiple Origins of Human Neocortical Interneurons Are Supported by Distinct Expression of Transcription Factors. Cereb Cortex : (2010). IHC-Fr ; Human . Read more (PubMed: 21139075) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human fibroblast and stem cells)
Loading amount 50 µg
Specification human fibroblast and stem cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Username

Abcam user community

Verified customer

Submitted Sep 10 2012

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Thank you also for the image that you have provided me with, it is most useful in understanding the difficulties that you have experienced. As...

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Thank you for your enquiry. I am sorry to hear you are having a problem with ab22885 (LHX6 antibody). To improve the detection of LHX6, I would like to suggest the following modifications to your protocol: 1) As LHX6 is a nuclear protein, use RI...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (brain, fixed in acrolein)
Specification brain, fixed in acrolein
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 6%
Username

Miss. Nina Hasen

Verified customer

Submitted Jun 01 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"