Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab45983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 40 kDa).Can be blocked with LHX6 peptide (ab45982).
IHC-FoFr 1/1000.

Target

  • FunctionProbable transcription factor required for the expression of a subset of genes involved in interneurons migration and development. Functions in the specification of cortical interneuron subtypes and in the migration of GABAergic interneuron precursors from the subpallium to the cerebral cortex.
  • Sequence similaritiesContains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • LHX 6 antibody
    • LHX 6.1 antibody
    • LHX6 antibody
    • LHX6.1 antibody
    • LHX6_HUMAN antibody
    • LIM homeobox 6 antibody
    • LIM homeobox protein 6 antibody
    • LIM homeodomain protein 6.1 antibody
    • LIM/homeobox protein Lhx6 antibody
    • LIM/homeobox protein Lhx6.1 antibody
    • MGC119542 antibody
    • MGC119544 antibody
    • MGC119545 antibody
    • OTTHUMP00000064113 antibody
    • OTTHUMP00000064114 antibody
    see all

Anti-LHX6 antibody images

  • Anti-LHX6 antibody (ab45983) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 40 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa,72 kDa. We are unsure as to the identity of these extra bands.
  • ab45983 staining LHX6 in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary.

    See Abreview

References for Anti-LHX6 antibody (ab45983)

This product has been referenced in:
  • Aligny C  et al. Ketamine alters cortical integration of GABAergic interneurons and induces long-term sex-dependent impairments in transgenic Gad67-GFP mice. Cell Death Dis 5:e1311 (2014). WB ; Mouse . Read more (PubMed: 24991763) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Jun 12 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"