This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

  • Product name
  • Description
    Rabbit polyclonal to Lhx8

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Host species
    Rabbit
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Lhx8.

    (Peptide available as ab45116.)

  • Positive control
    • This antibody gave a positive signal in the following Tissue Lysates: Mouse Liver Rat Liver Mouse Brain (data not shown)

Properties

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 39 kDa.

Target

Images

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Anti-Lhx8 antibody (ab41519) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate - normal tissue
    Lane 2 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 39 kDa
    Observed band size: 43 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Shi J  et al. Lhx8 promote differentiation of hippocampal neural stem/progenitor cells into cholinergic neurons in vitro. In Vitro Cell Dev Biol Anim 48:603-9 (2012). IHC . Read more (PubMed: 23150137) »
  • White YA  et al. Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women. Nat Med 18:413-21 (2012). Read more (PubMed: 22366948) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Embryonic day 19.5 brain tissue)
Permeabilization
No
Specification
Embryonic day 19.5 brain tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 12 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (neuroblastoma)
Loading amount
300000 cells
Specification
neuroblastoma
Gel Running Conditions
Non-reduced Denaturing (12% Bis-Tris)
Blocking step
Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: RT°C
Username

Ann-Christin Schmädicke

Verified customer

Submitted Jan 17 2012

Vielen Dank für Ihre Email und den Abreview über ab41519. Diese Informationen sind sehr wichtig für andere Forscher und wir schätzen Ihr Feedback sehr. Der Gutscheincode wird gültig sobald ein Abreview eingereicht ist. Ihr Gutscheincode ist somit ab ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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