This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

Properties

Applications

  • Application notesThis antibody gave a positive result in ELISA against the immunizing peptide (ab45117).
    Not yet tested in other applications.

    Optimal dilutions/concentrations should be determined by the end user.

  • Target

    Anti-Lhx8 antibody images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • All lanes : Anti-Lhx8 antibody (ab45118) at 1 µg/ml

      Lane 1 : Liver (Mouse) Tissue Lysate
      Lane 2 : Liver (Rat) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 25 kDa,40 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-Lhx8 antibody (ab45118)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - whole (Ms Liver, Ms Kidney, Rat Liver, Rat Kidney)
    Loading amount 20 µg
    Specification Ms Liver, Ms Kidney, Rat Liver, Rat Kidney
    Gel Running Conditions Reduced Denaturing (Precise protein gel 4-20%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Aug 12 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"