Overview

  • Product nameAnti-LHX9 antibody
    See all LHX9 primary antibodies
  • Description
    Rabbit polyclonal to LHX9
  • Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Zebrafish
  • Immunogen

    A region within a synthetic peptide: NLLRQENGGV DKADGTSLPA PPSADSGALT PPGTATTLTD LTNPTITVVT, corresponding to C terminal amino acids 325-374 of Human LHX9

  • Positive control
    • Jurkat cell lysate, human muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab28735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-LHX9 antibody images

  • Anti-LHX9 antibody (ab28735) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg/ml

    Secondary
    HRP conjugated anti-Rabbit IgG

    Predicted band size : 44 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of LHX9 expression in paraffin embedded human muscle tissue section using 6 µg/ml ab28735. Arrows indicate positively labelled skeletal muscle cells.

References for Anti-LHX9 antibody (ab28735)

ab28735 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"