Overview

  • Product name
  • Description
    Rabbit polyclonal to LHX9
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: HNPDAKDLKQ LAQKTGLTKR VLQVWFQNAR AKFRRNLLRQ ENGGVDKADG, corresponding to C terminal amino acids 289-338 of Human LHX9

  • Positive control
    • WB: Jurkat cell lysate. IHC-P: Human intestine tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab28737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/62500.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-LHX9 antibody (ab28737) at 1.25 µg/ml + Jurkat cell lysate at 10 µg/ml

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)
  • Ab28737, at a dilution of 4.0-8.0 µg/ml, staining LHX9 in paraffin embedded human intestine tissue by Immunohistochemistry. The arrows point to positively labelled epithelial cells of the intestinal villus.
    Magnification: 400X.

References

This product has been referenced in:
  • Vladimirova V  et al. Aberrant methylation and reduced expression of LHX9 in malignant gliomas of childhood. Neoplasia 11:700-11 (2009). WB ; Human . Read more (PubMed: 19568415) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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