Recombinant
RabMAb

Anti-LI Cadherin antibody [EPR3997] (ab109220)

Overview

  • Product name
    Anti-LI Cadherin antibody [EPR3997]
    See all LI Cadherin primary antibodies
  • Description
    Rabbit monoclonal [EPR3997] to LI Cadherin
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to the C terminus of Human LI Cadherin.

  • Positive control
    • WB: Human small intestine and colon cancer lysates IHC-P: Human colon and colonic adenocarcinoma tissues
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR3997
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.
    • Tissue specificity
      Expressed in the gastrointestinal tract and pancreatic duct. Not detected in kidney, lung, liver, brain, adrenal gland and skin.
    • Sequence similarities
      Contains 7 cadherin domains.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • BILL-cadherin antibody
      • CAD17_HUMAN antibody
      • Cadherin 16 antibody
      • Cadherin 16, formerly antibody
      • Cadherin 17 antibody
      • cadherin 17, LI cadherin (liver-intestine) antibody
      • cadherin, liver-intestine antibody
      • Cadherin-17 antibody
      • CDH16 antibody
      • CDH16, formerly antibody
      • Cdh17 antibody
      • FLJ26931 antibody
      • Formerly Cadherin 16 antibody
      • Formerly CDH16 antibody
      • HPT 1 antibody
      • HPT-1 cadherin antibody
      • human intestinal peptide-associated transporter HPT-1 antibody
      • human peptide transporter 1 antibody
      • Intestinal peptide-associated transporter HPT 1 antibody
      • Intestinal peptide-associated transporter HPT-1 antibody
      • LI-cadherin antibody
      • Liver Cadherin antibody
      • liver intestine cadherin antibody
      • Liver-intestine cadherin antibody
      see all

    Images

    • All lanes : Anti-LI Cadherin antibody [EPR3997] (ab109220) at 1/1000 dilution

      Lane 1 : Human small intestine cancer lysates
      Lane 2 : Colon cancer lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 92 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    • ab109220, at a 1/250 dilution, staining LI Cadherin in paraffin embedded Human colonic adenocarcinoma tissue by Immunohistochemistry.
    • ab109220, at a 1/250 dilution, staining LI Cadherin in paraffin embedded Human colon tissue by Immunohistochemistry.
    • ab109220 showing negative staining in Normal liver tissue.

    • ab109220 showing positive staining in Normal colon tissue.

    • ab109220 showing negative staining in Normal tonsil tissue.

    • ab109220 showing negative staining in Papillary carcinoma of thyroid gland tissue.

    • ab109220 showing negative staining in Normal kidney tissue.

    • ab109220 showing negative staining in Normal brain tissue.

    • ab109220 showing negative staining in Prostatic carcinoma tissue.

    • ab109220 showing negative staining in Breast carcinoma tissue.

    References

    This product has been referenced in:
    • Li R  et al. Inhibition of CDH17 gene expression via RNA interference reduces proliferation and apoptosis of human MKN28 gastric cancer cells. Int J Oncol 50:15-22 (2017). IHC-P ; Human . Read more (PubMed: 27909714) »
    • Yu J  et al. High-throughput proteomics integrated with gene microarray for discovery of colorectal cancer potential biomarkers. Oncotarget 7:75279-75292 (2016). IHC-P ; Human . Read more (PubMed: 27661117) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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