Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab34427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 20 kDa).

Target

  • Function
    LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
  • Sequence similarities
    Belongs to the LIF/OSM family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDF antibody
    • Cholinergic Differentiation Factor antibody
    • D factor antibody
    • DIA antibody
    • Differentiation inducing factor antibody
    • differentiation inhibitory activity antibody
    • Differentiation stimulating factor antibody
    • Differentiation-stimulating factor antibody
    • Emfilermin antibody
    • Hepatocyte stimulating factor III antibody
    • HILDA antibody
    • Human interleukin in DA cells antibody
    • Leukemia inhibitory factor antibody
    • LIF antibody
    • LIF_HUMAN antibody
    • Melanoma derived LPL inhibitor antibody
    • Melanoma-derived LPL inhibitor antibody
    • MLPLI antibody
    see all

Images

  • All lanes : Anti-LIF antibody [4F7.2] (ab34427) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 87 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References

This product has been referenced in:
  • Youngblood BA  et al. Application of recombinant human leukemia inhibitory factor (LIF) produced in rice (Oryza sativa L.) for maintenance of mouse embryonic stem cells. J Biotechnol 172:67-72 (2014). WB . Read more (PubMed: 24380819) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A431)
Loading amount
30 µg
Specification
A431
Gel Running Conditions
Non-reduced Denaturing (7.5% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Raghavendra Karamsetty

Verified customer

Submitted Nov 01 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Mature astroglial culture)
Specification
Mature astroglial culture
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 15 2012

Thank you for your enquiry. The concentration is 1 mg/ml. I hope this information helps. Please do not hesitate to contact us again if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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