Overview

  • Product nameLIF Human ELISA KitSee all LIF kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.41 84% - 103%
    Serum 94.26 83% - 106%
    Plasma 93.64 82% - 102%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s LIF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LIF in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human LIF coated on a 96-well plate. Standards and samples are pipetted into the wells and LIF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LIF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LIF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

     

    LIF (Leukocyte inhibitory factor) is produced by various fibroblast cell lines, antigen-stimulated alloreactive T-lymphocytes, mitogen-activated spleen cells, and Krebs and Ehrlich ascites cells. It is also produced by monocytes after cell activation. This factor inhibits the random and directed migration of polymorphonuclear (PMN) leukocytes. LIF also induces specific granule secretion by PMNs and potentiates many responses mediated by the chemotactic compound fMLP (formyl-methionyl-leucylphenylalanine).

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionLIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
  • Sequence similaritiesBelongs to the LIF/OSM family.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P15018 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • CDF
    • Cholinergic differentiation factor
    • D factor
    • DIA
    • Differentiation inhibitory activity
    • Differentiation stimulating factor
    • Differentiation-stimulating factor
    • Emfilermin
    • Hepatocyte stimulating factor III
    • HILDA
    • Human interleukin in DA cells
    • Leukemia inhibitory factor
    • LIF
    • LIF_HUMAN
    • Melanoma derived LPL inhibitor
    • Melanoma-derived LPL inhibitor
    • MLPLI
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

LIF Human ELISA Kit images

  • Representative Standard Curve using ab100582

  • Representative Standard Curve using ab100582

Protocols

References for LIF Human ELISA Kit (ab100582)

ab100582 has not yet been referenced specifically in any publications.

Product Wall

Siento no haber contestado antes, me temo que no tengo la información de todos los productos solicitados, sin embargo, puedo proporcionarte acerca de los siguientes kits:

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"