Anti-LIM domain only 3 antibody (ab230490)
Key features and details
- Rabbit polyclonal to LIM domain only 3
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-LIM domain only 3 antibody -
Description
Rabbit polyclonal to LIM domain only 3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse, Rat, Cow, Zebrafish, Orangutan -
Immunogen
Synthetic peptide corresponding to Human LIM domain only 3 aa 50-150.
Database link: Q8TAP4 -
Positive control
- WB: COS-7 cell extracts. IHC-P: Human testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)
Mg2+ and Ca2+ free. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000.
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IHC-P |
Use at an assay dependent dilution.
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Notes |
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WB
1/500 - 1/1000. |
IHC-P
Use at an assay dependent dilution. |
Target
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Sequence similarities
Contains 2 LIM zinc-binding domains. - Information by UniProt
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Database links
- Entrez Gene: 55885 Human
- Entrez Gene: 109593 Mouse
- Entrez Gene: 553774 Zebrafish
- Omim: 180386 Human
- SwissProt: Q8TAP4 Human
- SwissProt: Q8BZL8 Mouse
- SwissProt: Q5RBW7 Orangutan
- SwissProt: Q99MB5 Rat
see all -
Alternative names
- DAT 1 antibody
- DAT1 antibody
- LIM domain only 3 (rhombotin like 2) antibody
see all
Images
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All lanes : Anti-LIM domain only 3 antibody (ab230490) at 1/500 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 2 : COS-7 cell extract with Immunizing peptide
Developed using the ECL technique. -
Formalin-fixed, paraffin-embedded human testis tissue stained for LIM domain only 3 with ab230490 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab230490 has been referenced in 1 publication.
- Fernandes HJR et al. Single-Cell Transcriptomics of Parkinson's Disease Human In Vitro Models Reveals Dopamine Neuron-Specific Stress Responses. Cell Rep 33:108263 (2020). PubMed: 33053338