Overview

  • Product name
    Anti-LIM Kinase 1 antibody
    See all LIM Kinase 1 primary antibodies
  • Description
    Rabbit polyclonal to LIM Kinase 1
  • Host species
    Rabbit
  • Specificity
    ab39641 detects endogenous levels of total LIM Kinase 1 protein.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide (non-phosphorylated) derived from human LIM Kinase 1 around the phosphorylation site of Threonine 508.

  • Positive control
    • A2780 and NIH/3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab39641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
ELISA
  • Application notes
    ELISA: 1/4000.
    ICC/IF: 1/100 (PMID 19208621).
    WB: 1/500 - 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
    • Tissue specificity
      Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
    • Involvement in disease
      Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
    • Sequence similarities
      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 2 LIM zinc-binding domains.
      Contains 1 PDZ (DHR) domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated.
      Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
      Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM.
    • Cellular localization
      Cytoplasm. Cell projection > growth cone.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • EC 2.7.1.37 antibody
      • LIM domain containing protein kinase antibody
      • LIM domain kinase 1 antibody
      • LIM kinase antibody
      • LIM motif containing protein kinase antibody
      • LIMK 1 antibody
      • LIMK antibody
      • LIMK-1 antibody
      • limk1 antibody
      • LIMK1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-LIM Kinase 1 antibody (ab39641)
      Western blot - Anti-LIM Kinase 1 antibody (ab39641)
      All lanes : Anti-LIM Kinase 1 antibody (ab39641) at 1/500 dilution

      Lane 1 : NIH/3T3 cell extracts
      Lane 2 : A2780 cell extracts

      Predicted band size: 73 kDa
      Observed band size: 73 kDa



      5-30µg of cell extract was loaded per lane.
    • Immunocytochemistry/ Immunofluorescence - Anti-LIM Kinase 1 antibody (ab39641)
      Immunocytochemistry/ Immunofluorescence - Anti-LIM Kinase 1 antibody (ab39641)Image from Montani L et al, J Biol Chem. 2009 Apr 17;284(16):10793-807. Epub 2009 Feb 9, Fig 5, DOI 10.1074/jbc.M808297200.
      ab39641 staining LIM Kinase 1 in the growth cones of Human DRG neurons by Immunocytochemistry/ Immunofluorescence.
      Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature and permeabilized (0.1% Triton X-100 and 1× phosphate-buffered saline). After blocking, cells were incubated for double staining with primary antibodies, followed by incubation with the appropriate Cy3-secondary antibody for 30 minutes and with 4',6-diamidino-2-phenylindole. F-actin staining with Alexa 488-phalloidin.

      Scale bar = 10µm.

    References

    This product has been referenced in:
    • Zhou Y  et al. DADS downregulates the Rac1-ROCK1/PAK1-LIMK1-ADF/cofilin signaling pathway, inhibiting cell migration and invasion. Oncol Rep 29:605-12 (2013). Read more (PubMed: 23233092) »
    • Tondeleir D  et al. Cells lacking ß-actin are genetically reprogrammed and maintain conditional migratory capacity. Mol Cell Proteomics : (2012). Read more (PubMed: 22448045) »

    See all 3 Publications for this product

    Publishing research using ab39641? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

    10
    Votes: Highest First

    I'm desperate for a LIMK1 antibody for use in Drosophila, but have been unable to find a product that has been tested in flies. Looking at the fly LIMK1 sequence, the region around the phosphorylation site is fairly well conserved. So: 1) Could you ...

    Read More

    Thank you for your enquiry. Following a sequence alignment; http://www.ebi.ac.uk/cgi-bin/jobresults/clustalw/clustalw-20061214-15265852.aln? It appears that there is a degree of sequence similarity between the Drosophila and Human homologs....

    Read More

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