Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)
- Datasheet
- Specific References (8)
- Protocols
Overview
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Product nameAnti-LIM Kinase 1 (phospho T508) antibody
See all LIM Kinase 1 primary antibodies -
DescriptionRabbit polyclonal to LIM Kinase 1 (phospho T508)
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Host speciesRabbit
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SpecificityThis antibody is specific for LIM Kinase only when phosphorylated at threonine 508. It recognizes both LIMK1 and LIMK2 from human, mouse, and rat.
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Tested applicationsSuitable for: WB, IHC-P, ELISA, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic phosphopeptide derived from Human LIM Kinase around the phosphorylation site of threonine 508.
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Positive control
- IHC-P: Human breast carcinoma tissue slides. WB: COLO and MCF7 cell extracts.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab38508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 73 kDa). | |
| IHC-P | 1/50 - 1/100. | |
| ELISA | 1/10000. | |
| ICC/IF | 1/100. PubMed: 19208621 |
Target
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FunctionProtein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes.
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Tissue specificityHighest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
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Involvement in diseaseNote=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
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Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated.
Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM. -
Cellular localizationCytoplasm. Cell projection > growth cone.
- Information by UniProt
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Database links
- Entrez Gene: 3984 Human
- Entrez Gene: 16885 Mouse
- Entrez Gene: 65172 Rat
- Omim: 601329 Human
- SwissProt: P53667 Human
- SwissProt: P53668 Mouse
- SwissProt: P53669 Rat
- Unigene: 647035 Human
see all -
Alternative names
- EC 2.7.1.37 antibody
- LIM domain containing protein kinase antibody
- LIM domain kinase 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)ab38508 at a 1:50 dilution staining LIM Kinase in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.Left image : untreated.
Right image : treated with phosphopeptide.
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Western blot - Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)All lanes : Anti-LIM Kinase 1 (phospho T508) antibody (ab38508) at 1/500 dilution
Lane 1 : Extract of COLO cells + peptide.
Lane 2 : Extract of COLO cells.
Lane 3 : Extract of MCF7 cells.
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)
Predicted band size: 73 kDa
Observed band size: 65 kDa (why is the actual band size different from the predicted?)
Lanes can be loaded with 5-30µg of total protein. The arrow next to the image points to LIM Kinase (phospho T508). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 (phospho T508) antibody (ab38508)Image from Mokalled MH et al, Development. 2010 Jul;137(14):2365-74. Epub 2010 Jun 9, Fig 6. DOI: 10.1242/dev.047605.ab38508 staining LIM Kinase 1 in mouse brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
bdKO mice were anesthetized and transcardially perfused with PBS, followed by 4% paraformaldehyde (PFA) prior to brain dissection. Brains were then postfixed in 4% PFA for 2 days, embedded in paraffin and sectioned.
Protocols
References
This product has been referenced in:
- Tsai CS et al. Thrombomodulin regulates monocye differentiation via PKCd and ERK1/2 pathway in vitro and in atherosclerotic artery. Sci Rep 6:38421 (2016). WB ; Human . Read more (PubMed: 27910925) »
- Ray S et al. LIM kinase regulation of cytoskeletal dynamics is required for salivary gland branching morphogenesis. Mol Biol Cell 25:2393-407 (2014). Read more (PubMed: 24966172) »
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