Anti-LIM kinase 2 antibody (ab70116)

Overview

  • Product nameAnti-LIM kinase 2 antibody
    See all LIM kinase 2 primary antibodies
  • Description
    Rabbit polyclonal to LIM kinase 2
  • Specificityab70116 detects endogenous levels of total LIM kinase 2 protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human LIM kinase 2

  • Positive control
    • HeLa cells, HepG2 and HUVEC cell extracts and human heart tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab70116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
ELISA 1/40000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionDisplays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
  • Tissue specificityHighest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on serine and/or threonine residues by ROCK1.
  • Cellular localizationCytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • LIM domain kinase 2 antibody
    • LIMK 2 antibody
    • LIMK-2 antibody
    • Limk2 antibody
    • LIMK2_HUMAN antibody
    see all

Anti-LIM kinase 2 antibody images

  • ab70116 at 1/500 dilution staining LIM kinase 2 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • ab70116 at 1/50 dilution staining LIM kinase 2 in human heart by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-LIM kinase 2 antibody (ab70116) at 1/500 dilution

    Lane 1 : HepG2 cell extracts
    Lane 2 : HUVEC cell extracts
    Lane 3 : HepG2 cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 72 kDa
    Observed band size : 72 kDa

References for Anti-LIM kinase 2 antibody (ab70116)

ab70116 has not yet been referenced specifically in any publications.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"