Overview

  • Product nameAnti-LIM kinase 2 antibody
    See all LIM kinase 2 primary antibodies
  • Description
    Rabbit polyclonal to LIM kinase 2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment with a sequence corresponding to a region within amino acids 294 and 665 of Human LIM kinase 2 (AAH13051).

  • Positive control
    • Molt-4 and Raji cell lines. 293T whole cell lysate. Paraffin-embedded CA922 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab97766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 78 kDa.
IHC-P 1/100 - 1/500.

Target

  • FunctionDisplays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
  • Tissue specificityHighest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on serine and/or threonine residues by ROCK1.
  • Cellular localizationCytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • LIM domain kinase 2 antibody
    • LIMK 2 antibody
    • LIMK-2 antibody
    • Limk2 antibody
    • LIMK2_HUMAN antibody
    see all

Anti-LIM kinase 2 antibody images

  • Anti-LIM kinase 2 antibody (ab97766) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 78 kDa
  • ab97766, at 1/500, staining paraffin-embedded CA922 xenograft by Immunohistochemistry.

References for Anti-LIM kinase 2 antibody (ab97766)

ab97766 has not yet been referenced specifically in any publications.

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