Overview

  • Product nameAnti-LIM kinase 2 antibody
    See all LIM kinase 2 primary antibodies
  • Description
    Rabbit polyclonal to LIM kinase 2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1 - 252 of Human LIM kinase 2

  • Positive control
    • 293T, A431, H1299, HeLa S3, HepG2, MOLT4 and Raji cell lines; A549 cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab97773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 72 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionDisplays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
  • Tissue specificityHighest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on serine and/or threonine residues by ROCK1.
  • Cellular localizationCytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • LIM domain kinase 2 antibody
    • LIMK 2 antibody
    • LIMK-2 antibody
    • Limk2 antibody
    • LIMK2_HUMAN antibody
    see all

Anti-LIM kinase 2 antibody images

  • All lanes : Anti-LIM kinase 2 antibody (ab97773) at 1/2000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 72 kDa
  • ab97773, at a 1/200 dilution, staining LIM kinase 2 in paraformaldehyde fixed A549 cells by Immunofluorescence analysis. The lower image (panel 2) was merged with DNA probe.

References for Anti-LIM kinase 2 antibody (ab97773)

ab97773 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab97773.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"