Anti-LIM kinase 2 antibody [EP969Y] (ab45165)

Overview

  • Product name
    Anti-LIM kinase 2 antibody [EP969Y]
    See all LIM kinase 2 primary antibodies
  • Description
    Rabbit monoclonal [EP969Y] to LIM kinase 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IF, Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of human LIM kinase 2.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab45165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
WB 1/2000. Predicted molecular weight: 72 kDa.

Target

  • Function
    Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
  • Tissue specificity
    Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
  • Sequence similarities
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on serine and/or threonine residues by ROCK1.
  • Cellular localization
    Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • LIM domain kinase 2 antibody
    • LIMK 2 antibody
    • LIMK-2 antibody
    • Limk2 antibody
    • LIMK2_HUMAN antibody
    see all

Images

  • Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
    Lane 2: LIMK2 (LIM kinase 2) knockout HAP1 whole cell lysate (40 µg)
    Lane 3: Jurkat whole cell lysate (40 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab45165 observed at 72 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab45165 was shown to specifically recognize LIMK2 (LIM kinase 2) in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed with knockout cells were examined. Wild-type and LIMK2 (LIM kinase 2) knockout samples were subjected to SDS-PAGE. Ab45165 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

  • ab45165 staining human LIM kinase 2 in human ovary carcinoma by immunohistochemistry using paraffin embedded tissue.

  • Anti-LIM kinase 2 antibody [EP969Y] (ab45165) at 1/2000 dilution + Ramos membrane

    Predicted band size: 72 kDa
    Observed band size: 70 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemical analyis of mouse upper eyelid tissue, staining LIM kinase 2 with ab45165 at 1/200 dilution. Staining was detected using DAB.

References

This product has been referenced in:
  • Hyun HW  et al. Mitochondrial Translocation of High Mobility Group Box 1 Facilitates LIM Kinase 2-Mediated Programmed Necrotic Neuronal Death. Front Cell Neurosci 10:99 (2016). Read more (PubMed: 27147971) »
  • Ko AR  et al. Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal death independent of NOS activity. Mol Brain 8:58 (2015). IHC-FoFr ; Rat . Read more (PubMed: 26438559) »

See all 5 Publications for this product

Customer reviews and Q&As

Es freut mich Ihnen mitteilen zu können, dass wir die Konzentration des ab45165 Anti-LIM kinase 2 antibody [EP969Y] doch bestimmt haben: Für Ihr Lot beträgt sie 0.0390 mg/ml.

The concentration is 0.0390 mg/ml.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP (prostate cancer cell line))
Loading amount
10 µg
Specification
LNCaP (prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing (7% tris-acetate gel)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jul 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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