Anti-LIM kinase 2 antibody [EP969Y] (ab45165)

Overview

• Product name
  Anti-LIM kinase 2 antibody [EP969Y]
  See all LIM kinase 2 primary antibodies
• Description
  Rabbit monoclonal [EP969Y] to LIM kinase 2
• Tested applications
  Suitable for: IHC-P, ICC/IF, Flow Cyt, IP, WBmore details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to residues near the C-terminus of human LIM kinase 2.

• General notes

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage buffer
  PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Purity
  Tissue culture supernatant
• Clonality
  Monoclonal
• Clone number
  EP969Y
• Isotype
  IgG
• Research areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

Applications

Target

• Function
  Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
• Tissue specificity
  Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
• Sequence similarities
  Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
  Contains 2 LIM zinc-binding domains.
  Contains 1 PDZ (DHR) domain.
  Contains 1 protein kinase domain.
• Post-translational
  modifications
  Phosphorylated on serine and/or threonine residues by ROCK1.
• Cellular localization
  Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.

• Target information above from: UniProt accession P53671 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 3985 Human
  • Entrez Gene: 16886 Mouse
  • Entrez Gene: 29524 Rat
  • Omim: 601988 Human
  • SwissProt: P53671 Human
  • SwissProt: O54785 Mouse
  • SwissProt: P53670 Rat
  • Unigene: 474596 Human

  • Unigene: 124176 Mouse
  • Unigene: 390323 Mouse
  • Unigene: 442189 Mouse
  • Unigene: 11013 Rat

  see all
• Alternative names
  • LIM domain kinase 2 antibody
  • LIMK 2 antibody
  • LIMK-2 antibody

  • Limk2 antibody
  • LIMK2_HUMAN antibody

  see all