- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameAnti-LIM kinase 2 antibody [EP969Y]
See all LIM kinase 2 primary antibodies -
DescriptionRabbit monoclonal [EP969Y] to LIM kinase 2
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cyt, IP, WBmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to residues near the C-terminus of human LIM kinase 2.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP969Y
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab45165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | 1/50 - 1/100. | |
| Flow Cyt | 1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IP | 1/50. | |
| WB | 1/2000. Predicted molecular weight: 72 kDa. |
Target
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FunctionDisplays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
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Tissue specificityHighest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
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Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated on serine and/or threonine residues by ROCK1. -
Cellular localizationCytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 3985 Human
- Entrez Gene: 16886 Mouse
- Entrez Gene: 29524 Rat
- Omim: 601988 Human
- SwissProt: P53671 Human
- SwissProt: O54785 Mouse
- SwissProt: P53670 Rat
- Unigene: 474596 Human
see all -
Alternative names
- LIM domain kinase 2 antibody
- LIMK 2 antibody
- LIMK-2 antibody
see all
Images
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![Western blot - Anti-LIM kinase 2 antibody [EP969Y] (ab45165)](http://a.abcam.com/ps/products/45/ab45165/Images/ab45165-289257-ab45165GDBKOWB.jpg)
Western blot - Anti-LIM kinase 2 antibody [EP969Y] (ab45165)Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
Lane 2: LIMK2 (LIM kinase 2) knockout HAP1 whole cell lysate (40 µg)
Lane 3: Jurkat whole cell lysate (40 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab45165 observed at 72 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab45165 was shown to specifically recognize LIMK2 (LIM kinase 2) in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed with knockout cells were examined. Wild-type and LIMK2 (LIM kinase 2) knockout samples were subjected to SDS-PAGE. Ab45165 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM kinase 2 antibody [EP969Y] (ab45165)](http://a.abcam.com/ps/products/45/ab45165/Images/ab45165_2.bmp)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM kinase 2 antibody [EP969Y] (ab45165)ab45165 staining human LIM kinase 2 in human ovary carcinoma by immunohistochemistry using paraffin embedded tissue.
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![Western blot - Anti-LIM kinase 2 antibody [EP969Y] (ab45165)](http://a.abcam.com/ps/products/45/ab45165/Images/ab45165_1.bmp)
Western blot - Anti-LIM kinase 2 antibody [EP969Y] (ab45165)Anti-LIM kinase 2 antibody [EP969Y] (ab45165) at 1/2000 dilution + Ramos membrane
Predicted band size: 72 kDa
Observed band size: 70 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM kinase 2 antibody [EP969Y] (ab45165)](http://a.abcam.com/ps/products/45/ab45165/Images/LIM-kinase-2-Primary-antibodies-ab45165-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM kinase 2 antibody [EP969Y] (ab45165)Image from Rice DS et al., PLoS One. 2012;7(10):e47168. doi: 10.1371/journal.pone.0047168. Epub 2012 Oct 5.; Fig 6.; October 5, 2012, PLoS ONE 7(10): e47168.Immunohistochemical analyis of mouse upper eyelid tissue, staining LIM kinase 2 with ab45165 at 1/200 dilution. Staining was detected using DAB.
References
This product has been referenced in:
- Hyun HW et al. Mitochondrial Translocation of High Mobility Group Box 1 Facilitates LIM Kinase 2-Mediated Programmed Necrotic Neuronal Death. Front Cell Neurosci 10:99 (2016). Read more (PubMed: 27147971) »
- Ko AR et al. Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal death independent of NOS activity. Mol Brain 8:58 (2015). IHC-FoFr ; Rat . Read more (PubMed: 26438559) »
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