Anti-LIM kinase 2 antibody [EP969Y] (ab45165)

Overview

  • Product nameAnti-LIM kinase 2 antibody [EP969Y]
    See all LIM kinase 2 primary antibodies
  • Description
    Rabbit monoclonal [EP969Y] to LIM kinase 2
  • Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of human LIM kinase 2.

  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab45165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
WB 1/2000. Predicted molecular weight: 72 kDa.

Target

  • FunctionDisplays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
  • Tissue specificityHighest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on serine and/or threonine residues by ROCK1.
  • Cellular localizationCytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • LIM domain kinase 2 antibody
    • LIMK 2 antibody
    • LIMK-2 antibody
    • Limk2 antibody
    • LIMK2_HUMAN antibody
    see all

Anti-LIM kinase 2 antibody [EP969Y] images

  • Anti-LIM kinase 2 antibody [EP969Y] (ab45165) at 1/2000 dilution + Ramos membrane

    Predicted band size : 72 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • An45165 staining human LIM kinase 2 in human ovary carcinoma by immunohistochemistry using paraffin embedded tissue.
  • Immunohistochemical analyis of mouse upper eyelid tissue, staining LIM kinase 2 with ab45165 at 1/200 dilution. Staining was detected using DAB.

References for Anti-LIM kinase 2 antibody [EP969Y] (ab45165)

This product has been referenced in:
  • Ko AR  et al. Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal death independent of NOS activity. Mol Brain 8:58 (2015). IHC-FoFr ; Rat . Read more (PubMed: 26438559) »
  • Ray S  et al. LIM kinase regulation of cytoskeletal dynamics is required for salivary gland branching morphogenesis. Mol Biol Cell 25:2393-407 (2014). Read more (PubMed: 24966172) »

See all 4 Publications for this product

Product Wall

Es freut mich Ihnen mitteilen zu können, dass wir die Konzentration des ab45165 Anti-LIM kinase 2 antibody [EP969Y] doch bestimmt haben: Für Ihr Lot beträgt sie 0.0390 mg/ml.

The concentration is 0.0390 mg/ml.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (LNCaP (prostate cancer cell line))
Loading amount 10 µg
Specification LNCaP (prostate cancer cell line)
Gel Running Conditions Reduced Denaturing (7% tris-acetate gel)
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jul 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"