Overview

  • Product nameAnti-LIM kinase 2b antibody
  • Description
    Rabbit polyclonal to LIM kinase 2b
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human LIM kinase 2b.

  • Positive control
    • This antibody gave a positive signal in Saos2 whole cell lysate and Human Brain tissue lysate. This antibody gave a positive signal in the following cell types (ICC/IF): Hek293.

Properties

Applications

Our Abpromise guarantee covers the use of ab93854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).

Target

  • FunctionDisplays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
  • Tissue specificityHighest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
  • Sequence similaritiesBelongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 2 LIM zinc-binding domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on serine and/or threonine residues by ROCK1.
  • Cellular localizationCytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • LIM containing protein kinase 2b antibody
    • LIM domain kinase 2 antibody
    • LIM domain kinase 2b antibody
    • LIM kinase 2b antibody
    • LIM motif containing protein kinase 2b antibody
    • LIMK 2b antibody
    • LIMK-2 antibody
    • Limk2 antibody
    • LIMK2 b antibody
    • LIMK2_HUMAN antibody
    • LIMK2b antibody
    see all

Anti-LIM kinase 2b antibody images

  • All lanes : Anti-LIM kinase 2b antibody (ab93854) at 1 µg/ml

    Lane 1 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
    Lane 2 : Human brain tissue lysate - total protein (ab29466)
    Lane 3 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 4 : Human brain tissue lysate - total protein (ab29466) with Immunising peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 72 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 56 kDa,62 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab93854 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab93854 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-LIM kinase 2b antibody (ab93854)

ab93854 has not yet been referenced specifically in any publications.

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