Overview

  • Product nameAnti-LIM1 antibody
    See all LIM1 primary antibodies
  • Description
    Rabbit polyclonal to LIM1
  • Specificityab22861 recognises LIM1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Chimpanzee, Zebrafish
  • Immunogen

    A region within a synthetic peptide: EPSLPGPLHS MSAEVFGPSP PFSSLSVNGG ASYGNHLSHP PEMNEAAVW, corresponding to C terminal amino acids 358-456 of Human LIM1

  • Positive control
    • HepG2 cell lysate and Human liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab22861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.4 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionPotential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis.
  • Tissue specificityExpressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines.
  • Sequence similaritiesContains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • DomainThe LIM domains exert a negative regulatory function and disruption of the LIM domains produces an activated form. In addition, two activation domains and a negative regulatory domain exist C-terminally to the homeobox.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hLim-1 antibody
    • Homeo box protein Lim 1 antibody
    • Homeo box protein Lim1 antibody
    • Homeobox protein Lim 1 antibody
    • Homeobox protein Lim-1 antibody
    • Homeobox protein Lim1 antibody
    • LHX 1 antibody
    • LHX1 antibody
    • LHX1_HUMAN antibody
    • LIM 1 antibody
    • LIM homeo box 1 antibody
    • LIM homeo box protein 1 antibody
    • LIM homeobox 1 antibody
    • LIM homeobox protein 1 antibody
    • LIM-1 antibody
    • LIM/homeobox protein Lhx 1 antibody
    • LIM/homeobox protein Lhx1 antibody
    • MGC126723 antibody
    • MGC138141 antibody
    see all

Anti-LIM1 antibody images

  • Anti-LIM1 antibody (ab22861) at 0.4 µg/ml + HepG2 cell lysate. with 5% skim milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
  • Immunohistochemistry of Human Liver cell lysate tissue at an antibody concentration of 4-8µg/ml using anti-LHX1 antibody (ab22861)

References for Anti-LIM1 antibody (ab22861)

ab22861 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your e-mail and for your very interesting comment. I have just checked our file and talked to my colleagues and would like to confirm that the peptide position was wrong. It should be 356-406 instead of 356-410. I have already amended...

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The antibody has been tested in formalin fixed paraffin embedded tissue but not yet in frozen sections. In our hands, the signal is specific to the tissue we expect to cross react with. We usually this antibody with 9 standard tissues as well as in pa...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"