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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa).
Function Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis.
Tissue specificity Expressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines.
Sequence similarities Contains 1 homeobox DNA-binding domain. Contains 2 LIM zinc-binding domains.
Domain The LIM domains exert a negative regulatory function and disruption of the LIM domains produces an activated form. In addition, two activation domains and a negative regulatory domain exist C-terminally to the homeobox.
Cellular localization Nucleus.
Information by UniProt
Homeo box protein Lim 1 antibody
Homeo box protein Lim1 antibody
Anti-LIM1 antibody images
Western blot - LIM1 antibody (ab84789)
Anti-LIM1 antibody (ab84789) at 1 µg/ml + Human thymus tissue lysate - total protein ( ab30146) at 10 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 44 kDa Observed band size : 42 kDa ( why is the actual band size different from the predicted?) Exposure time : 4 minutes
References for Anti-LIM1 antibody (ab84789)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"