Overview

  • Product nameAnti-LIM1 antibody
    See all LIM1 primary antibodies
  • Description
    Rabbit polyclonal to LIM1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Hamster, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human LIM1.

  • Positive control
    • This antibody gave a positive signal in Human thymus tissue lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab84789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa).

Target

  • FunctionPotential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis.
  • Tissue specificityExpressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines.
  • Sequence similaritiesContains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • DomainThe LIM domains exert a negative regulatory function and disruption of the LIM domains produces an activated form. In addition, two activation domains and a negative regulatory domain exist C-terminally to the homeobox.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hLim-1 antibody
    • Homeo box protein Lim 1 antibody
    • Homeo box protein Lim1 antibody
    • Homeobox protein Lim 1 antibody
    • Homeobox protein Lim-1 antibody
    • Homeobox protein Lim1 antibody
    • LHX 1 antibody
    • LHX1 antibody
    • LHX1_HUMAN antibody
    • LIM 1 antibody
    • LIM homeo box 1 antibody
    • LIM homeo box protein 1 antibody
    • LIM homeobox 1 antibody
    • LIM homeobox protein 1 antibody
    • LIM-1 antibody
    • LIM/homeobox protein Lhx 1 antibody
    • LIM/homeobox protein Lhx1 antibody
    • MGC126723 antibody
    • MGC138141 antibody
    see all

Anti-LIM1 antibody images

  • Anti-LIM1 antibody (ab84789) at 1 µg/ml + Human thymus tissue lysate - total protein (ab30146) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

References for Anti-LIM1 antibody (ab84789)

ab84789 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"