Anti-LIMCH1 antibody (ab96178)
Key features and details
- Rabbit polyclonal to LIMCH1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-LIMCH1 antibody -
Description
Rabbit polyclonal to LIMCH1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment, corresponding to a sequence within amino acids 734-1057 of Human LIMCH1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 119 kDa.
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IHC-P | (1) |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Suggest antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/3000. Predicted molecular weight: 119 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggest antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Target
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Sequence similarities
Belongs to the LIMCH1 family.
Contains 1 CH (calponin-homology) domain.
Contains 1 LIM zinc-binding domain. - Information by UniProt
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Database links
- Entrez Gene: 22998 Human
- Entrez Gene: 77569 Mouse
- SwissProt: Q9UPQ0 Human
- SwissProt: Q3UH68 Mouse
- Unigene: 335163 Human
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Alternative names
- DKFZp434I0312 antibody
- DKFZp686A01247 antibody
- DKFZp686B2470 antibody
see all
Images
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Anti-LIMCH1 antibody (ab96178) at 1/2000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 119 kDaRun on 5% SDS PAGE gel
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Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ab96178 antibody at 1:500 dilution.
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Anti-LIMCH1 antibody (ab96178) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 119 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab96178 has been referenced in 4 publications.
- Cao H et al. Loss of LIMCH1 predicts poor prognosis in patients with surgically resected Lung Adenocarcinoma: A study based on Immunohistochemical Analysis and Bioinformatics. J Cancer 12:181-189 (2021). PubMed: 33391414
- Sarvestani SK et al. Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity. Nat Commun 12:262 (2021). PubMed: 33431859
- Wang Y et al. Circular RNA circLDB2 functions as a competing endogenous RNA to suppress development and promote cisplatin sensitivity in non-squamous non-small cell lung cancer. Thorac Cancer 12:1959-1972 (2021). PubMed: 34096174
- Bichell TJV et al. Reduced bioavailable manganese causes striatal urea cycle pathology in Huntington's disease mouse model. Biochim Biophys Acta 1863:1596-1604 (2017). PubMed: 28213125