Overview

  • Product name
  • Description
    Rabbit polyclonal to LIMD1
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Platypus
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human LIMD1 (NP_055055.1).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab114104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    Images

    • Detection of Human LIMD1 by Western blot of Immunoprecipitate.
      LIMD1 was immunoprecipitated from HeLa whole cell lysate, using ab114104 at 6 µg/mg lysate (1 mg for IP, 20% of IP loaded). An affinity purified rabbit anti-LIMD1 antibody was used for the subsequent WB at 0.4 µg/ml.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    References

    ab114104 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 22 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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