Overview

  • Product nameAnti-LIMD1 antibody
    See all LIMD1 primary antibodies
  • Description
    Rabbit polyclonal to LIMD1
  • Tested applicationsSuitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human LIMD1 (NP_055055.1).

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab114104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionTranscriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation.
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the zyxin/ajuba family.
      Contains 3 LIM zinc-binding domains.
    • Cellular localizationCytoplasm. Nucleus. Shuttles between cytoplasm and nucleus but is localized predominantly to the cytoplasm. Found in the nucleus but not nucleoli.
    • Information by UniProt
    • Database links
    • Alternative names
      • AW822033 antibody
      • D9Ertd192e antibody
      • LIM domain-containing protein 1 antibody
      • LIM domains containing 1 antibody
      • LIMD1 antibody
      • LIMD1_HUMAN antibody
      • OTTHUMP00000164657 antibody
      • OTTHUMP00000210317 antibody
      • rCG_25194 antibody
      see all

    Anti-LIMD1 antibody images

    • Detection of Human LIMD1 by Western blot of Immunoprecipitate.
      LIMD1 was immunoprecipitated from HeLa whole cell lysate, using ab114104 at 6 µg/mg lysate (1 mg for IP, 20% of IP loaded). An affinity purified rabbit anti-LIMD1 antibody was used for the subsequent WB at 0.4 µg/ml.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    References for Anti-LIMD1 antibody (ab114104)

    ab114104 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions Reduced Denaturing (10%)
    Loading amount 20 µg
    Specification HeLa
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    Submitted Sep 22 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"