Human Predicted to work with:
Recombinant fragment corresponding to amino acids 577-674 (VDSENKIYCV RDYHKVLAPK CAACGLPILP PEGSDETIRV VSMDRDYHVE CYHCEDCGLE LNDEDGHRCY PLEDHLFCHS CHVKRLEKRP SSTALHQH) of Human LIMD1 (NP_055055) with a proprietary tag.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 72 kDa.
Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining. Place sample in 1X citrate buffer (pH 6.0) in pressure cooker under 125oC for 4min and under 90oC for 45min.
FunctionTranscriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation.
Sequence similaritiesBelongs to the zyxin/ajuba family. Contains 3 LIM zinc-binding domains.
Cellular localizationCytoplasm. Nucleus. Shuttles between cytoplasm and nucleus but is localized predominantly to the cytoplasm. Found in the nucleus but not nucleoli.