Overview

  • Product nameAnti-LIMD1 antibody
    See all LIMD1 primary antibodies
  • Description
    Mouse monoclonal to LIMD1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to amino acids 577-674 (VDSENKIYCV RDYHKVLAPK CAACGLPILP PEGSDETIRV VSMDRDYHVE CYHCEDCGLE LNDEDGHRCY PLEDHLFCHS CHVKRLEKRP SSTALHQH) of Human LIMD1 (NP_055055) with a proprietary tag.

  • Positive control
    • Human pancreas tissue, LIMD1 transfected 293T cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab118367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 72 kDa.
IHC-P Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining. Place sample in 1X citrate buffer (pH 6.0) in pressure cooker under 125oC for 4min and under 90oC for 45min.

Target

  • FunctionTranscriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the zyxin/ajuba family.
    Contains 3 LIM zinc-binding domains.
  • Cellular localizationCytoplasm. Nucleus. Shuttles between cytoplasm and nucleus but is localized predominantly to the cytoplasm. Found in the nucleus but not nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW822033 antibody
    • D9Ertd192e antibody
    • LIM domain-containing protein 1 antibody
    • LIM domains containing 1 antibody
    • LIMD1 antibody
    • LIMD1_HUMAN antibody
    • OTTHUMP00000164657 antibody
    • OTTHUMP00000210317 antibody
    • rCG_25194 antibody
    see all

Anti-LIMD1 antibody images

  • Anti-LIMD1 antibody (ab118367) at 5 µg/ml + recombinant protein (immunogen) at 0.2 µg

    Predicted band size : 72 kDa
  • All lanes : Anti-LIMD1 antibody (ab118367) at 5 µg/ml

    Lane 1 : LIMD1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 72 kDa
  • Formalin-fixed paraffin-embedded Human pancreas tissue labelled with ab118367 at 3 µg/ml.

References for Anti-LIMD1 antibody (ab118367)

ab118367 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"