Overview

  • Product name
    Anti-LIMD1 antibody - C-terminal
    See all LIMD1 primary antibodies
  • Description
    Rabbit polyclonal to LIMD1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within C terminal amino acids 472-676 of Human LIMD1 (Uniprot ID Q9UGP4).

  • Positive control
    • 293T and Mouse brain whole cell lysates; A431, H1299, HeLaS3 and MOLT4 cell lines; OVCA xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 72 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Transcriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the zyxin/ajuba family.
    Contains 3 LIM zinc-binding domains.
  • Cellular localization
    Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus but is localized predominantly to the cytoplasm. Found in the nucleus but not nucleoli.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW822033 antibody
    • D9Ertd192e antibody
    • LIM domain-containing protein 1 antibody
    • LIM domains containing 1 antibody
    • LIMD1 antibody
    • LIMD1_HUMAN antibody
    • OTTHUMP00000164657 antibody
    • OTTHUMP00000210317 antibody
    • rCG_25194 antibody
    see all

Images

  • Anti-LIMD1 antibody - C-terminal (ab155788) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 72 kDa



    12% SDS PAGE
  • Anti-LIMD1 antibody - C-terminal (ab155788) at 1/500 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 72 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded OVCA xenograft, labeling LIMD1 with ab155788 at 1/300 dilution.

References

ab155788 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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