Overview

  • Product nameAnti-LIME antibody
    See all LIME primary antibodies
  • Description
    Rabbit polyclonal to LIME
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 189-219 of Human LIME (NP_060276.2).

  • Positive control
    • CEM cell line lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab123308 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab123308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 31 kDa.

Target

  • RelevanceLIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.
  • Cellular localizationCell membrane; Single-pass type III membrane protein. Present in lipid rafts. Recruited to the immunological synapse upon conjugation of T cell with antigen presenting cell
  • Database links
  • Alternative names
    • dJ583P15.4 antibody
    • FLJ20406 antibody
    • Lck interacting membrane protein antibody
    • Lck interacting molecule antibody
    • Lck interacting transmembrane adaptor 1 antibody
    • LIME 1 antibody
    • LIME1 antibody
    • LP8067 antibody
    • RP4 583P15.5 antibody
    see all

Anti-LIME antibody images

  • Anti-LIME antibody (ab123308) at 1/100 dilution + CEM cell line lysates at 35 µg

    Predicted band size : 31 kDa

References for Anti-LIME antibody (ab123308)

ab123308 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"