Recombinant
RabMAb

Anti-LIME antibody [EPR14713] (ab188871)

Overview

  • Product name
    Anti-LIME antibody [EPR14713]
    See all LIME primary antibodies
  • Description
    Rabbit monoclonal [EPR14713] to LIME
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human LIME aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9H400

  • Positive control
    • HuT-78 and Human thymus lysates; Human tonsil and mammary gland infiltrating duct carcinoma tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14713
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab188871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance
    LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.
  • Cellular localization
    Cell membrane; Single-pass type III membrane protein. Present in lipid rafts. Recruited to the immunological synapse upon conjugation of T cell with antigen presenting cell
  • Database links
  • Alternative names
    • dJ583P15.4 antibody
    • FLJ20406 antibody
    • Lck interacting membrane protein antibody
    • Lck interacting molecule antibody
    • Lck interacting transmembrane adaptor 1 antibody
    • LIME 1 antibody
    • LIME1 antibody
    • LP8067 antibody
    • RP4 583P15.5 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human mammary gland infiltrating duct carcinoma tissue labeling LIME with ab188871 at 1/2000 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling LIME with ab188871 at 1/2000 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

  • Anti-LIME antibody [EPR14713] (ab188871) at 1/1000 dilution + Human thymus lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution

    Predicted band size : 31 kDa
  • Anti-LIME antibody [EPR14713] (ab188871) at 1/10000 dilution + HuT-78 lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 31 kDa

References

ab188871 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab188871.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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