Anti-LIME antibody [LIME-06] (Phycoerythrin) (ab64678)

Overview

  • Product name
    Anti-LIME antibody [LIME-06] (Phycoerythrin)
    See all LIME primary antibodies
  • Description
    Mouse monoclonal [LIME-06] to LIME (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to aa 141-295 of human LIME.

  • Positive control
    • Peripheral blood T cells stimulated with anti-human CD4.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Size exclusion
  • Purification notes
    Purified antibody (>95% by SDS-PAGE) was conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate was purified by size-exclusion chromatography.
  • Clonality
    Monoclonal
  • Clone number
    LIME-06
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 5 µg/ml.

Target

  • Relevance
    LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.
  • Cellular localization
    Cell membrane; Single-pass type III membrane protein. Present in lipid rafts. Recruited to the immunological synapse upon conjugation of T cell with antigen presenting cell
  • Database links
  • Alternative names
    • dJ583P15.4 antibody
    • FLJ20406 antibody
    • Lck interacting membrane protein antibody
    • Lck interacting molecule antibody
    • Lck interacting transmembrane adaptor 1 antibody
    • LIME 1 antibody
    • LIME1 antibody
    • LP8067 antibody
    • RP4 583P15.5 antibody
    see all

References

ab64678 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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