Anti-LIMPII antibody (ab87637)

Overview

  • Product name
  • Description
    Goat polyclonal to LIMPII
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide:

    C-NKANIQFGDNGTTIS

    , corresponding to internal sequence amino acids 96-110 of human LIMPII (NP_005497.1).

  • Positive control
    • Human brain (cortex and cerebellum) lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab87637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 54 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Acts as a lysosomal receptor for glucosylceramidase (GBA) targeting.
    (Microbial infection) Acts as a receptor for enterovirus 71.
  • Involvement in disease
    Epilepsy, progressive myoclonic 4, with or without renal failure
    Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease.
  • Sequence similarities
    Belongs to the CD36 family.
  • Cellular localization
    Lysosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 85 kDa lysosomal membrane sialoglycoprotein antibody
    • 85 kDa lysosomal sialoglycoprotein scavenger receptor class B member 2 antibody
    • AMRF antibody
    • CD36 antibody
    • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2 (lysosomal integral membrane protein II) antibody
    • CD36 antigen antibody
    • CD36 antigen-like 2 antibody
    • CD36L2 antibody
    • EPM4 antibody
    • HLGP85 antibody
    • LGP85 antibody
    • LIMP 2 antibody
    • LIMP II antibody
    • LIMP2 antibody
    • LIMPII antibody
    • Lysosomal integral membrane protein II antibody
    • Lysosome membrane protein 2 antibody
    • Lysosome membrane protein II antibody
    • OTTHUMP00000160590 antibody
    • OTTHUMP00000219176 antibody
    • Scarb2 antibody
    • Scavenger receptor class B member 2 antibody
    • Scavenger receptor class B, member 2 antibody
    • SCRB2_HUMAN antibody
    • SR BII antibody
    • SRBII antibody
    see all

Images

  • Immunohistochemical analysis of Paraformaldehyde perfusion-fixed frozen human neuroblastoma xenograft into a maurine host. The image is labelling LIMPII with ab87637 at 20°C for 1 hour at a dilution of 1/50 with 5% Donkey Serum. Permeabilization was done with 0.2% Triton. Secondary used was ab96935 at a 1/250 dilution.

    See Abreview

  • Anti-LIMPII antibody (ab87637) at 0.1 µg/ml + human cerebral cortex lysate (in RIPA buffer) at 35 µg

    Predicted band size : 54 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • ab87637 (3.8µg/ml) staining of paraffin embedded Human Cerebral Cortex shows lysosomal staining. Tissue pretreated using steamed antigen retrieval with citrate buffer pH 6 followed by AP-staining.

References

ab87637 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain bearing human tumor)
Antigen retrieval step
None
Permeabilization
Yes - triton 0.2%
Specification
Brain bearing human tumor
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Mrs. Francoise Geffroy

Verified customer

Submitted Oct 26 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U87)
Permeabilization
Yes - TRITON 0.2%
Specification
U87
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Mrs. Francoise Geffroy

Verified customer

Submitted Oct 25 2015

Thank you for contacting us. The antibody ab87637 has not been tested in either cytochemistry or histochemistry. I assume you want to stain cell cultures or tissue sections, followed by IF detection. The antibody has not been validated on either sa...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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