Anti-LIMPII antibody (ab87637)

Overview

  • Product nameAnti-LIMPII antibody
    See all LIMPII primary antibodies
  • Description
    Goat polyclonal to LIMPII
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide:

    C-NKANIQFGDNGTTIS

    , corresponding to internal sequence amino acids 96-110 of human LIMPII (NP_005497.1).

  • Positive control
    • Human brain (cortex and cerebellum) lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab87637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 54 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionActs as a lysosomal receptor for glucosylceramidase (GBA) targeting.
    (Microbial infection) Acts as a receptor for enterovirus 71.
  • Involvement in diseaseEpilepsy, progressive myoclonic 4, with or without renal failure
    Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease.
  • Sequence similaritiesBelongs to the CD36 family.
  • Cellular localizationLysosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 85 kDa lysosomal membrane sialoglycoprotein antibody
    • 85 kDa lysosomal sialoglycoprotein scavenger receptor class B member 2 antibody
    • AMRF antibody
    • CD36 antibody
    • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2 (lysosomal integral membrane protein II) antibody
    • CD36 antigen antibody
    • CD36 antigen-like 2 antibody
    • CD36L2 antibody
    • EPM4 antibody
    • HLGP85 antibody
    • LGP85 antibody
    • LIMP 2 antibody
    • LIMP II antibody
    • LIMP2 antibody
    • LIMPII antibody
    • Lysosomal integral membrane protein II antibody
    • Lysosome membrane protein 2 antibody
    • Lysosome membrane protein II antibody
    • OTTHUMP00000160590 antibody
    • OTTHUMP00000219176 antibody
    • Scarb2 antibody
    • Scavenger receptor class B member 2 antibody
    • Scavenger receptor class B, member 2 antibody
    • SCRB2_HUMAN antibody
    • SR BII antibody
    • SRBII antibody
    see all

Anti-LIMPII antibody images

  • Immunohistochemical analysis of Paraformaldehyde perfusion-fixed frozen human neuroblastoma xenograft into a maurine host. The image is labelling LIMPII with ab87637 at 20°C for 1 hour at a dilution of 1/50 with 5% Donkey Serum. Permeabilization was done with 0.2% Triton. Secondary used was ab96935 at a 1/250 dilution.

    See Abreview

  • Anti-LIMPII antibody (ab87637) at 0.1 µg/ml + human cerebral cortex lysate (in RIPA buffer) at 35 µg

    Predicted band size : 54 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • ab87637 (3.8µg/ml) staining of paraffin embedded Human Cerebral Cortex shows lysosomal staining. Tissue pretreated using steamed antigen retrieval with citrate buffer pH 6 followed by AP-staining.

References for Anti-LIMPII antibody (ab87637)

ab87637 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain bearing human tumor)
Antigen retrieval step None
Permeabilization Yes - triton 0.2%
Specification Brain bearing human tumor
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative Paraformaldehyde
Username

Ms. Francoise Geffroy

Verified customer

Submitted Oct 26 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U87)
Permeabilization Yes - TRITON 0.2%
Specification U87
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Ms. Francoise Geffroy

Verified customer

Submitted Oct 25 2015

Thank you for contacting us. The antibody ab87637 has not been tested in either cytochemistry or histochemistry. I assume you want to stain cell cultures or tissue sections, followed by IF detection. The antibody has not been validated on either sa...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"