Recombinant Anti-LIMPII antibody [EPR12080] (ab176317)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12080] to LIMPII
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-LIMPII antibody [EPR12080]
See all LIMPII primary antibodies -
Description
Rabbit monoclonal [EPR12080] to LIMPII -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human LIMPII aa 50-150. The exact sequence is proprietary.
Database link: Q14108 -
Positive control
- WB: HeLa and Y79 cell lysate. Rat eyeball and muscle lysate. Mouse liver lysate. IP: Y79 cell lysate. Flow Cyt (intra): Permeabilized HeLa cells. IHC-P:Human kidney and liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12080 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 54 kDa.
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IP |
1/10 - 1/100.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 54 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Acts as a lysosomal receptor for glucosylceramidase (GBA) targeting.
(Microbial infection) Acts as a receptor for enterovirus 71. -
Involvement in disease
Epilepsy, progressive myoclonic 4, with or without renal failure
Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease. -
Sequence similarities
Belongs to the CD36 family. -
Cellular localization
Lysosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 950 Human
- Entrez Gene: 12492 Mouse
- Entrez Gene: 117106 Rat
- Omim: 602257 Human
- SwissProt: Q14108 Human
- SwissProt: O35114 Mouse
- SwissProt: P27615 Rat
- Unigene: 349656 Human
see all -
Alternative names
- 85 kDa lysosomal membrane sialoglycoprotein antibody
- 85 kDa lysosomal sialoglycoprotein scavenger receptor class B member 2 antibody
- AMRF antibody
see all
Images
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All lanes : Anti-LIMPII antibody [EPR12080] (ab176317) at 1/1000 dilution
Lane 1 : Wild-type MCF7 cell lysate
Lane 2 : SCARB2 knockout MCF7 cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-LIMPII antibody [EPR12080] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab176317 was shown to bind specifically to LIMPII. A band was observed at 80 kDa in wild-type MCF7 cell lysates with no signal observed at this size in SCARB2 knockout cell line ab274952 (knockout cell lysate ab275010). To generate this image, wild-type and SCARB2 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-LIMPII antibody [EPR12080] (ab176317) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate
Lane 2 : Y79 (Human retinoblastoma cell line) lysate
Lane 3 : Rat eyeball lysate
Lane 4 : Rat muscle lysate
Lane 5 : Mouse liver lysate
Predicted band size: 54 kDa -
Western blot analysis on immunoprecipitation pellet from Y79 (Human retinoblastoma cell line) cell lysate labeling LIMPII using ab176317 at a 1/10 dilution.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue labling LIMPII using ab176317 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human liver tissue labling LIMPII using ab176317 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of permeabilized HeLa cells labeling LIMPII using ab176317 at a 1/10 dilution (red)or a rabbit IgG control (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab176317 has been referenced in 7 publications.
- Yeo H et al. A Single Amino Acid Substitution in Structural Protein VP2 Abrogates the Neurotropism of Enterovirus A-71 in Mice. Front Microbiol 13:821976 (2022). PubMed: 35369482
- Eriksson I et al. Restoration of lysosomal function after damage is accompanied by recycling of lysosomal membrane proteins. Cell Death Dis 11:370 (2020). PubMed: 32409651
- Hase K et al. Cytosolic domain of SIDT2 carries an arginine-rich motif that binds to RNA/DNA and is important for the direct transport of nucleic acids into lysosomes. Autophagy 16:1974-1988 (2020). PubMed: 31944164
- Birket MJ et al. A Human Stem Cell Model of Fabry Disease Implicates LIMP-2 Accumulation in Cardiomyocyte Pathology. Stem Cell Reports 13:380-393 (2019). PubMed: 31378672
- Conrad KS et al. Lysosomal integral membrane protein-2 as a phospholipid receptor revealed by biophysical and cellular studies. Nat Commun 8:1908 (2017). PubMed: 29199275
- Aizawa S et al. Lysosomal membrane protein SIDT2 mediates the direct uptake of DNA by lysosomes. Autophagy 13:218-222 (2017). WB ; Human . PubMed: 27846365
- Aizawa S et al. Lysosomal putative RNA transporter SIDT2 mediates direct uptake of RNA by lysosomes. Autophagy 12:565-78 (2016). PubMed: 27046251