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Rabbit polyclonal to LIMS1
Predicted to work with:
Mouse, Rat, Chicken, Zebrafish, Xenopus tropicalis
Recombinant fragment, corresponding to a region within amino acids 1-216 of Human LIMS1 (UniProt: P48059).
HeLa, HCT116 and A549 whole cell lysates; Human BT474 xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 37 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Effector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway.
Expressed in most tissues except in the brain.
Contains 5 LIM zinc-binding domains.
Cell junction > focal adhesion. Cell membrane.
Information by UniProt
Western blot - Anti-LIMS1 antibody (ab154331)
Anti-LIMS1 antibody (ab154331) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 37 kDa 10% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMS1 antibody (ab154331)
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, labeling LIMS1 with ab154331 at 1/100 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"