Overview

  • Product nameAnti-LIMS1 antibody
    See all LIMS1 primary antibodies
  • Description
    Rabbit polyclonal to LIMS1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Cow, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human LIMS1.

    (Peptide available as ab88119.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysate: A549

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab78814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionEffector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway.
  • Tissue specificityExpressed in most tissues except in the brain.
  • Sequence similaritiesContains 5 LIM zinc-binding domains.
  • Cellular localizationCell junction > focal adhesion. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2310016J22Rik antibody
    • 4921524A02Rik antibody
    • AI507642 antibody
    • AU021743 antibody
    • AW551584 antibody
    • C430041B13Rik antibody
    • LIM and senescent cell antigen like domains 1 antibody
    • LIM and senescent cell antigen-like-containing domain protein 1 antibody
    • LIM zinc finger domain containing 1 antibody
    • LIM-type zinc finger domains 1 antibody
    • Lims1 antibody
    • LIMS1_HUMAN antibody
    • Lims1l antibody
    • Particularly interesting new Cys His protein 1 antibody
    • Particularly interesting new Cys His protein antibody
    • Particularly interesting new Cys-His protein 1 antibody
    • PINCH 1 antibody
    • PINCH antibody
    • PINCH-1 antibody
    • PINCH1 antibody
    • Renal carcinoma antigen NY REN 48 antibody
    • Renal carcinoma antigen NY-REN-48 antibody
    • senescent cell antigen antibody
    • wu:fc32c03 antibody
    • zgc:112533 antibody
    see all

Anti-LIMS1 antibody images

  • Anti-LIMS1 antibody (ab78814) at 1 µg/ml + A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa


    Exposure time : 15 minutes
  • ICC/IF image of ab78814 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78814, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-LIMS1 antibody (ab78814)

ab78814 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"