Anti-Lin28 antibody [6D1F9] (ab76369)

Overview

  • Product nameAnti-Lin28 antibody [6D1F9]
    See all Lin28 primary antibodies
  • Description
    Mouse monoclonal [6D1F9] to Lin28
  • Tested applicationsSuitable for: Sandwich ELISA, WB, ELISA, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant tagged fragment, corresponding to amino acids 93-209 of Human Lin28 expressed in E. coli strain BL21 (DE3)

  • Positive control
    • Truncated Trx-Lin28 (aa93-209) recombinant protein. Lin28 (aa93-209) tagged recombinant fragment. Full length Lin28-hIgGFc transfected CHOK1 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab76369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Lin28 (ab63740). For sandwich ELISA, use this antibody as Capture at 0.2 µg/ml with Rabbit polyclonal to Lin28 (ab63740) as Detection.
WB 1/500 - 1/2000. Predicted molecular weight: 23 kDa.
ELISA 1/10000.
ICC/IF 1/200 - 1/1000.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionActs as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
  • Tissue specificityExpressed in embryonic stem cells (ES cells), placenta and testis.
  • Sequence similaritiesBelongs to the lin-28 family.
    Contains 2 CCHC-type zinc fingers.
    Contains 1 CSD (cold-shock) domain.
  • Developmental stageExpressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
  • DomainThe CSD domain is required for function in muscle differentiation.
  • Cellular localizationCytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • AL024421 antibody
    • CSDD1 antibody
    • FLJ12457 antibody
    • Lin 28 antibody
    • Lin 28 homolog (C. elegans) antibody
    • Lin 28 homolog A (C. elegans) antibody
    • Lin 28 homolog A antibody
    • Lin 28 homolog antibody
    • Lin-28A antibody
    • Lin28 antibody
    • Lin28, C. elegans, homolog of, A antibody
    • LIN28A antibody
    • LN28A_HUMAN antibody
    • Protein lin-28 homolog A antibody
    • RNA binding protein lin 28 antibody
    • Tex17 antibody
    • ZCCHC1 antibody
    • Zinc finger CCHC domain containing 1 antibody
    • Zinc finger CCHC domain containing protein 1 antibody
    • Zinc finger CCHC domain-containing protein 1 antibody
    see all

Anti-Lin28 antibody [6D1F9] images

  • Standard Curve for Lin28 (Analyte: Lin28 protein (His tag) (ab89225)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [6D1F9] to Lin28 (ab76369) at 0.2 ug/ml and Detector Antibody Rabbit polyclonal to Lin28 (ab63740) at 0.1 ug/ml.
  • Immunofluorescence staining of methanol fixed HeLa cells were transfected with pMX construct of human Lin28. Cells were analyzed ~62 hours after transfection.
  • ab76369 at 1/2000 dillution staining Lin28 in NTERA-2 cells by Immunocytochemistry/ Immunofluorescence. The green color in image show positive staining with primary antibody while nuclei were stained blue with DRAQ5 fluorescent DNA dye.

  • Anti-Lin28 antibody [6D1F9] (ab76369) at 1/2000 dilution + Cell lysates prepared from human NTERA-2 cells

    Predicted band size : 23 kDa
  • Overlay histogram showing HeLa cells stained with ab76369 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76369, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

Protocols

References for Anti-Lin28 antibody [6D1F9] (ab76369)

ab76369 has not yet been referenced specifically in any publications.

Product Wall

Sorry for the delay in getting back to you. The concentration of the Anti-Lin28 antibody [6D1F9] (ab76369) is of the concentration 1 mg/mL for the lot which you have received.

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