Anti-Lin28A antibody (ab175352)
Key features and details
- Rabbit polyclonal to Lin28A
- Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Lin28A antibody
See all Lin28A primary antibodies -
Description
Rabbit polyclonal to Lin28A -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human Lin28A aa 1 to the C-terminus. Expressed in bacteria.
Database link: Q9H9Z2 -
Positive control
- WB: NTERA-2, NCCIT and HeLa whole cell lysates. ICC: H9 embryonic stem cells, HeLa cells, HEL 11.4 induced IPS cells, Human embryonal carcinoma NTERA-2 cells. IHC-P: Mouse heart tissue. Human tonsil and Human placenta tissues. Flow cyt: H9 embryonic stem cells, HEL 11.4 induced IPS cells. IP: NCCIT whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 30% Glycerol (glycerin, glycerine), 69% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/100.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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IHC-P |
1/20 - 1/50.
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WB |
1/1000. Predicted molecular weight: 22 kDa.
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ICC/IF |
1/100 - 1/200.
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IP |
1/1000.
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Notes |
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Flow Cyt
1/100. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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IHC-P
1/20 - 1/50. |
WB
1/1000. Predicted molecular weight: 22 kDa. |
ICC/IF
1/100 - 1/200. |
IP
1/1000. |
Target
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Function
Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. -
Tissue specificity
Expressed in embryonic stem cells (ES cells), placenta and testis. -
Sequence similarities
Belongs to the lin-28 family.
Contains 2 CCHC-type zinc fingers.
Contains 1 CSD (cold-shock) domain. -
Developmental stage
Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid. -
Domain
The CSD domain is required for function in muscle differentiation. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules. - Information by UniProt
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Database links
- Entrez Gene: 79727 Human
- Entrez Gene: 83557 Mouse
- Omim: 611043 Human
- SwissProt: Q9H9Z2 Human
- SwissProt: Q8K3Y3 Mouse
- Unigene: 86154 Human
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Alternative names
- CSDD1 antibody
- FLJ12457 antibody
- LIN 28 antibody
see all
Images
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All lanes : Anti-Lin28A antibody (ab175352) at 1/1000 dilution
Lane 1 : NCCIT whole cell lysate
Lane 2 : NTERA-2 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 75 µg per lane.
Secondary
All lanes : goat anti-rabbit-HRP secondary antibody at 1/20000 dilution
Predicted band size: 22 kDa -
Immunofluorescent analysis of formalin-fixed and permeabilized HeLa cells labeling Lin28A with ab175352 at 1/100 and incubated with a DyLight-conjugated secondary antibody. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.
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Immunofluorescent analysis of fH9 embryonic stem cells labeling Lin28A with ab175352 at 1/200 and incubated with a fluorescently-conjugated secondary antibody. Nuclei (blue) were stained with DAPI.
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Immunofluorescent analysis of HEL 11.4 induced IPS cells labeling Lin28A with ab175352 at 1/200 and incubated with a fluorescently-conjugated secondary antibody. Nuclei (blue) were stained with DAPI.
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Immunofluorescent analysis of formalin-fixed and permeabilized HeLa cells (left image) and Human embryonal carcinoma NTERA-2 cells (right image) labeling Lin28A with ab175352 at 1/200 and and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody. Nuclei (blue) were stained with Hoechst 33342 dye.
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Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Lin28A with ab175352 at 1/50.
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Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling Lin28A with ab175352 at 1/50.
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Flow Cytometrical analysis of H9 embryonic stem cells labeling Lin28A with ab175352 at 1/100 (right histogram) compared to rabbit IgGl (left histogram) and incubated with a fluorescently-conjugated secondary antibody.
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Paraffin embedded mouse heart tissue stained for Lin28A using ab175352 at 1/20 dilution in immunohistochemical analysis. DAB staining buffer was applied and tissues were counterstained with hematoxylin.
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Flow Cytometrical analysis of HEL 11.4 induced IPS cells labeling Lin28A with ab175352 at 1/100 (right histogram) compared to rabbit IgGl (left histogram) and incubated with a fluorescently-conjugated secondary antibody.
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Lane 2: Immunoprecipitation. ab175352 at 1/1000 labeling Lin28A immunoprecipitated from 750 μg of NCCIT whole cell lysate using ab175352 at 3μg.
Anti-Lin28A antibody (ab175352) at 1/1000 dilution + NCCIT whole cell lysate
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab175352 has not yet been referenced specifically in any publications.