Overview

  • Product name
  • Description
    Rabbit polyclonal to Lin28
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Lin28 aa 1-209. Expressed in bacteria.
    Database link: Q9H9Z2

  • Positive control
    • NTERA-2, NCCIT and HeLa whole cell lysates; H9 embryonic stem cells; HEL 11.4 induced IPS cells; Human tonsil and Human placenta tissues; Human embryonal carcinoma NTERA-2 cells; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab175352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/20 - 1/50.
WB 1/1000. Predicted molecular weight: 22 kDa.
ICC/IF 1/100 - 1/200.
IP 1/1000.

Target

  • Function
    Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
  • Tissue specificity
    Expressed in embryonic stem cells (ES cells), placenta and testis.
  • Sequence similarities
    Belongs to the lin-28 family.
    Contains 2 CCHC-type zinc fingers.
    Contains 1 CSD (cold-shock) domain.
  • Developmental stage
    Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
  • Domain
    The CSD domain is required for function in muscle differentiation.
  • Cellular localization
    Cytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • AL024421 antibody
    • CSDD1 antibody
    • CSDD2 antibody
    • FLJ12457 antibody
    • Lin 28 antibody
    • Lin 28 homolog (C. elegans) antibody
    • Lin 28 homolog A (C. elegans) antibody
    • Lin 28 homolog A antibody
    • Lin 28 homolog antibody
    • Lin-28A antibody
    • Lin28 antibody
    • Lin28, C. elegans, homolog of, A antibody
    • LIN28A antibody
    • LN28A_HUMAN antibody
    • Protein lin-28 homolog A antibody
    • Protein lin-28 homolog B antibody
    • RNA binding protein lin 28 antibody
    • Tex17 antibody
    • ZCCHC1 antibody
    • Zinc finger CCHC domain containing 1 antibody
    • Zinc finger CCHC domain containing protein 1 antibody
    • Zinc finger CCHC domain-containing protein 1 antibody
    see all

Images

  • All lanes : Anti-Lin28 antibody (ab175352) at 1/1000 dilution

    Lane 1 : NCCIT whole cell lysate
    Lane 2 : NTERA-2 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 75 µg per lane.

    Secondary
    goat anti-rabbit-HRP secondary antibody at 1/20000 dilution

    Predicted band size : 22 kDa
  • Immunofluorescent analysis of formalin-fixed and permeabilized HeLa cells labeling Lin28 with ab175352 at 1/100 and incubated with a DyLight-conjugated secondary antibody. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.

  • Immunofluorescent analysis of fH9 embryonic stem cells labeling Lin28 with ab175352 at 1/200  and incubated with a fluorescently-conjugated secondary antibody.  Nuclei (blue) were stained with DAPI.

  • Immunofluorescent analysis of HEL 11.4 induced IPS cells labeling Lin28 with ab175352 at 1/200 and incubated with a fluorescently-conjugated secondary antibody. Nuclei (blue) were stained with DAPI.

  • Immunofluorescent analysis of formalin-fixed and permeabilized HeLa cells (left image) and Human embryonal carcinoma NTERA-2 cells (right image) labeling Lin28 with ab175352 at 1/200 and and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody. Nuclei (blue) were stained with Hoechst 33342 dye.

  • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Lin28 with ab175352 at 1/50.

  • Immunohistochemical analysis of paraffin embedded Human placenta  tissue labeling Lin28 with ab175352 at 1/50.

  • Flow Cytometrical analysis of H9 embryonic stem cells labeling Lin28 with ab175352 at 1/100 (right histogram) compared to rabbit IgGl (left histogram) and incubated with a fluorescently-conjugated secondary antibody.

  • Flow Cytometrical analysis of HEL 11.4 induced IPS cells labeling Lin28 with ab175352 at 1/100 (right histogram) compared to rabbit IgGl (left histogram) and incubated with a fluorescently-conjugated secondary antibody.

  • Lane 2: Immunoprecipitation. ab175352 at 1/1000 labeling Lin28 immunoprecipitated from 750 μg of NCCIT whole cell lysate using ab175352 at 3μg.

References

ab175352 has not yet been referenced specifically in any publications.

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