Overview

  • Product nameAnti-Lin28 antibody
    See all Lin28 primary antibodies
  • Description
    Rabbit polyclonal to Lin28
  • Tested applicationsSuitable for: WB, IHC-P, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein (Human) with hexahistidine tag

Properties

Applications

Our Abpromise guarantee covers the use of ab46020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/7000. PubMed: 20101213
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionActs as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
  • Tissue specificityExpressed in embryonic stem cells (ES cells), placenta and testis.
  • Sequence similaritiesBelongs to the lin-28 family.
    Contains 2 CCHC-type zinc fingers.
    Contains 1 CSD (cold-shock) domain.
  • Developmental stageExpressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
  • DomainThe CSD domain is required for function in muscle differentiation.
  • Cellular localizationCytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • AL024421 antibody
    • CSDD1 antibody
    • FLJ12457 antibody
    • Lin 28 antibody
    • Lin 28 homolog (C. elegans) antibody
    • Lin 28 homolog A (C. elegans) antibody
    • Lin 28 homolog A antibody
    • Lin 28 homolog antibody
    • Lin-28A antibody
    • Lin28 antibody
    • Lin28, C. elegans, homolog of, A antibody
    • LIN28A antibody
    • LN28A_HUMAN antibody
    • Protein lin-28 homolog A antibody
    • RNA binding protein lin 28 antibody
    • Tex17 antibody
    • ZCCHC1 antibody
    • Zinc finger CCHC domain containing 1 antibody
    • Zinc finger CCHC domain containing protein 1 antibody
    • Zinc finger CCHC domain-containing protein 1 antibody
    see all

Anti-Lin28 antibody images

  • All lanes : Anti-Lin28 antibody (ab46020) at 1/1000 dilution

    Lane 1 : PC-3 cell extract in lysis buffer containing NP-40 at 45 µg
    Lane 2 : PC-3 cell extract (Trizol bottom phase) at 30 µg
    Lane 3 : Tera-2 cell extract (Trizol bottom phase) at 30 µg
    Lane 4 : Tera-2 cell extract in lysis buffer containing NP-40 at 45 µg

    Secondary
    Goat anti-rabbit HRP conjugated at 1/4000 dilution

    Predicted band size : 23 kDa
    Observed band size : 29 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.
    It is unclear why lin28 runs at a larger size than its predicted moleular weight but recombinant lin28 protein is also known to run at around 30 kDa.
  • Human Embryonic stem cells were fixed in PFA and then incubated with the primary antibody ab46020 (1:1000) diluted in 2% BSA for 16 hours at 4C. Cells where then washed and incubated with a Cy3 conjugated goat anti-rabbit polyclonal antibody (1:400). Cells where then washed and visualised.
  • ab46020 staining Lin28 in paraffin-embedded human tissue sections (ESC-derived embryoid bodies) by IHC-P. Tissue was fixed with paraformaldehyde and blocked with 8% Serum treatment in automated Bond Max system for 15 minutes. Samples were incubated with primary antibody (1/2000) for 15 minutes. An HRP-conjugated goat anti rabbit polyclonal (1/200) was used as secondary antibody.

    See Abreview

References for Anti-Lin28 antibody (ab46020)

This product has been referenced in:
  • Vessoni AT  et al. Cockayne syndrome-derived neurons display reduced synapse density and altered neural network synchrony. Hum Mol Genet 25:1271-80 (2016). Read more (PubMed: 26755826) »
  • Al-Lamki RS  et al. Tumor necrosis factor receptor 2-signaling in CD133-expressing cells in renal clear cell carcinoma. Oncotarget 7:24111-24 (2016). Human . Read more (PubMed: 26992212) »

See all 24 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryonic)
Permeabilization Yes - Triton X-100 (0.5%)
Specification Embryonic
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 23 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Human Cell lysate - whole cell (liver carcinoma cell line)
Specification liver carcinoma cell line
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 05 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse ES Cells)
Loading amount 50 µg
Specification Mouse ES Cells
Gel Running Conditions Reduced Denaturing (16% Gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Utkarsh Kapoor

Verified customer

Submitted Dec 28 2012

Thank you for contacting us yesterday.

As discussed over the phone, I have had a look into the two antibodies you have been using (ab46020 and ab71415).

As we discussed, the anti-Lin28 antibody (ab46020) has been raised against the ...

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Thank you again for your call yesterday and for your patience.



I've heard back from the lab about ab46020, and this antibody was raised against full-length Lin28A. It has not been tested specifically against Lin28B but since these ...

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Thank you for your call today and for letting us know about the trouble with ab46020. I apologize for the problems with this antibody and for the inconvenience.

As we discussed, I'm sending a free of charge vial of ab71415, which is specific ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (fetal ovary)
Specification fetal ovary
Fixative Bouin's fixative
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human fetal ovary)
Specification human fetal ovary
Fixative neutral buffered formalin
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Ovary)
Specification Ovary
Fixative Modified Davidson's Fixative
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization Yes - 0.1% Tween in TBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 18 2012

Thank you for contacting us. Ab46020 was raised against full length Isoform A. We predicted that it could cross-react with isoform B, however, we have not tested it in the laboratory. In our catalogue, there are totally 3 items which can specifically r...

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