• Product nameAnti-Lin28 antibody [EPR4639]
    See all Lin28 primary antibodies
  • Description
    Rabbit monoclonal [EPR4639] to Lin28
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of Human Lin28A.

  • Positive control
    • JAR and NCCIT cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab109751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionActs as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
    • Tissue specificityExpressed in embryonic stem cells (ES cells), placenta and testis.
    • Sequence similaritiesBelongs to the lin-28 family.
      Contains 2 CCHC-type zinc fingers.
      Contains 1 CSD (cold-shock) domain.
    • Developmental stageExpressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
    • DomainThe CSD domain is required for function in muscle differentiation.
    • Cellular localizationCytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
    • Information by UniProt
    • Database links
    • Alternative names
      • AL024421 antibody
      • CSDD1 antibody
      • FLJ12457 antibody
      • Lin 28 antibody
      • Lin 28 homolog (C. elegans) antibody
      • Lin 28 homolog A (C. elegans) antibody
      • Lin 28 homolog A antibody
      • Lin 28 homolog antibody
      • Lin-28A antibody
      • Lin28 antibody
      • Lin28, C. elegans, homolog of, A antibody
      • LIN28A antibody
      • LN28A_HUMAN antibody
      • Protein lin-28 homolog A antibody
      • RNA binding protein lin 28 antibody
      • Tex17 antibody
      • ZCCHC1 antibody
      • Zinc finger CCHC domain containing 1 antibody
      • Zinc finger CCHC domain containing protein 1 antibody
      • Zinc finger CCHC domain-containing protein 1 antibody
      see all

    Anti-Lin28 antibody [EPR4639] images

    • All lanes : Anti-Lin28 antibody [EPR4639] (ab109751) at 1/1000 dilution

      Lane 1 : JAR cell lysate
      Lane 2 : NCCIT cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 23 kDa

    References for Anti-Lin28 antibody [EPR4639] (ab109751)

    ab109751 has not yet been referenced specifically in any publications.

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