Overview

  • Product nameAnti-Lin28A antibody
    See all Lin28A primary antibodies
  • Description
    Rabbit polyclonal to Lin28A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide, corresponding to a region surrounding amino acids 1-5 (MGSVS) of Human Lin28A (NP_078950.1).

  • Positive control
    • HepG2 cell extract

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab119392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).

Target

  • FunctionActs as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
  • Tissue specificityExpressed in embryonic stem cells (ES cells), placenta and testis.
  • Sequence similaritiesBelongs to the lin-28 family.
    Contains 2 CCHC-type zinc fingers.
    Contains 1 CSD (cold-shock) domain.
  • Developmental stageExpressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
  • DomainThe CSD domain is required for function in muscle differentiation.
  • Cellular localizationCytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSDD1 antibody
    • FLJ12457 antibody
    • LIN 28 antibody
    • Lin 28 homolog A (C. elegans) antibody
    • Lin-28A antibody
    • LIN28 antibody
    • LIN28A antibody
    • LN28A_HUMAN antibody
    • Protein lin-28 homolog A antibody
    • ZCCHC1 antibody
    • zinc finger CCHC domain containing 1 antibody
    • Zinc finger CCHC domain-containing protein 1 antibody
    see all

Anti-Lin28A antibody images

  • Anti-Lin28A antibody (ab119392) at 1/500 dilution + HepG2 cell extract

    Predicted band size : 23 kDa

References for Anti-Lin28A antibody (ab119392)

ab119392 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"