Overview

  • Product nameAnti-Lin28A antibody [EPR4640]
    See all Lin28A primary antibodies
  • Description
    Rabbit monoclonal [EPR4640] to Lin28A
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    A synthetic peptide, corresponding to residues in Human Lin28A.

  • Positive control
    • NCCIT and JAR cell lysates; NCCIT cells; Human seminoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:
    Anti-Lin28A antibody (Alexa Fluor® 488) [EPR4640] (ab206079)
    Anti-Lin28A antibody (Alexa Fluor® 647) [EPR4640] (ab206263)

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR4640
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab124765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.

Target

  • FunctionActs as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
  • Tissue specificityExpressed in embryonic stem cells (ES cells), placenta and testis.
  • Sequence similaritiesBelongs to the lin-28 family.
    Contains 2 CCHC-type zinc fingers.
    Contains 1 CSD (cold-shock) domain.
  • Developmental stageExpressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
  • DomainThe CSD domain is required for function in muscle differentiation.
  • Cellular localizationCytoplasm. Nucleus > nucleolus. Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSDD1 antibody
    • FLJ12457 antibody
    • LIN 28 antibody
    • Lin 28 homolog A (C. elegans) antibody
    • Lin-28A antibody
    • LIN28 antibody
    • LIN28A antibody
    • LN28A_HUMAN antibody
    • Protein lin-28 homolog A antibody
    • ZCCHC1 antibody
    • zinc finger CCHC domain containing 1 antibody
    • Zinc finger CCHC domain-containing protein 1 antibody
    see all

Anti-Lin28A antibody [EPR4640] images

  • All lanes : Anti-Lin28A antibody [EPR4640] (ab124765) at 1/10000 dilution

    Lane 1 : NCCIT cell lysate
    Lane 2 : JAR cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size : 23 kDa
  • ab124765, at 1/100 dilution, staining Lin28A in paraffin-embedded Human seminoma tissue by Immunohistochemistry.
  • ab124765, at 1/100 dilution, staining Lin28A in NCCIT cells by Immunofluorescence.
  • ab124765 showing positive staining in Glioma tissue.

  • ab124765 showing positive staining in Dysgerminoma tissue.

  • ab124765 showing positive staining in Embryonal carcinoma tissue.

  • ab124765 showing negative staining in Normal brain tissue.

References for Anti-Lin28A antibody [EPR4640] (ab124765)

This product has been referenced in:
  • Liu H  et al. Single-cell clones of liver cancer stem cells have the potential of differentiating into different types of tumor cells. Cell Death Dis 4:e857 (2013). Flow Cyt ; Human . Read more (PubMed: 24136221) »

See 1 Publication for this product

Product Wall

Thank you for your email.

I am sorry for the confusion cased but this is difference is simply from an error on our part in setting up the new datasheet. The product should be stated as being against LIN28A. This will be updated as soon as pos...

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I am sorry for the delay in getting back to you.

I can now share further information with you in relation to the anti-Lin28A antibody [EPR4640] ab124765. This antibody is currently supplied at a concentration of 0.53 mg/mL. This is just withi...

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Thank you for your reply.

The conjugation kits we have available can be accessed from the following link. I was incorrect to think we had DyLight 488 available but we have FITC which may be a suitable alternative?

http://www.abcam.c...

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Thank you for contacting us.

I have good news as well as bad news. Good news is that this product will be available on Abcam website and bad news - it may take 1-2 weeks, 2 weeks will be maximum.

We have un-conjugated version ab12476...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"