Anti-LINE-1 ORF2p antibody (ab106004)
Key features and details
- Chicken polyclonal to LINE-1 ORF2p
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgY
Overview
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Product name
Anti-LINE-1 ORF2p antibody
See all LINE-1 ORF2p primary antibodies -
Description
Chicken polyclonal to LINE-1 ORF2p -
Host species
Chicken -
Specificity
The two synthetic immunogen peptides correspond to regions within the endonuclease domain of LINE-1 ORF2p protein. This antibody is intended for use in studying control of L1 retrotransposons.
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Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee -
Immunogen
Synthetic peptide corresponding to Human LINE-1 ORF2p conjugated to keyhole limpet haemocyanin. Two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of LINE-1 ORF2p protein.
Database link: O00370 -
Positive control
- induced bacterially expressed human ORF2
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General notes
Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening product.ab106004 is intended for use in studying control of L1 retrotransposons.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading...
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Purity
IgY fraction -
Purification notes
ab106004 is an IgY fraction antibody purified from monospecific Chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation, followed by extensive dialysis against the buffer. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 149 kDa.
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
1/2000 - 1/10000. Predicted molecular weight: 149 kDa. |
Target
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Database links
- SwissProt: O00370 Human
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Alternative names
- L1/ORF2 antibody
- LINE 1 ORF2p antibody
- LINE-1 retrotransposable element ORF2 protein antibody
see all
Images
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All lanes : Anti-LINE-1 ORF2p antibody (ab106004) at 1/2000 dilution
Lane 1 : induced bacterially expressed human ORF2
Lane 2 : uninduced bacterially expressed human ORF2
Predicted band size: 149 kDaNote: No specific band staining is seen in the uninduced lane (lane 2). The lower molecular weight bands represent non-specific staining. The band at ~70 kDa (arrowhead) corresponds to a Human LINE-1 ORF2p endonuclease domain fusion protein.
Datasheets and documents
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Datasheet download
References (4)
ab106004 has been referenced in 4 publications.
- Zhang H et al. Single-nucleus transcriptomic landscape of primate hippocampal aging. Protein Cell 12:695-716 (2021). PubMed: 34052996
- Bi S et al. SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer. Protein Cell 11:483-504 (2020). PubMed: 32504224
- Romero MA et al. Acute and chronic resistance training downregulates select LINE-1 retrotransposon activity markers in human skeletal muscle. Am J Physiol Cell Physiol 314:C379-C388 (2018). PubMed: 29351416
- Thomas CA et al. Modeling of TREX1-Dependent Autoimmune Disease using Human Stem Cells Highlights L1 Accumulation as a Source of Neuroinflammation. Cell Stem Cell 21:319-331.e8 (2017). PubMed: 28803918