Anti-LINE-1 retrotransposable element ORF2 protein antibody (ab106004)

Overview

  • Product name
    Anti-LINE-1 retrotransposable element ORF2 protein antibody
  • Description
    Chicken polyclonal to LINE-1 retrotransposable element ORF2 protein
  • Specificity
    The two synthetic immunogen peptides correspond to regions within the endonuclease domain of L1/ORF2 protein. This antibody is intended for use in studying control of L1 retrotransposons.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human LINE-1 retrotransposable element ORF2 protein conjugated to Keyhole Limpet Haemocyanin (KLH). Two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.
    Database link: O00370

  • Positive control
    • induced bacterially expressed human ORF2
  • General notes

    Dilute only prior to immediate use.
    Expiration date is one (1) year from date of opening product. 

    ab106004 is intended for use in studying control of L1 retrotransposons.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate
  • Concentration information loading...
  • Purity
    IgY fraction
  • Purification notes
    ab106004 is an IgY fraction antibody purified from monospecific Chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation, followed by extensive dialysis against the buffer.
  • Clonality
    Polyclonal
  • Isotype
    IgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 149 kDa.
ELISA 1/10000.

Images

  • All lanes : Anti-LINE-1 retrotransposable element ORF2 protein antibody (ab106004) at 1/2000 dilution

    Lane 1 : induced bacterially expressed human ORF2
    Lane 2 : uninduced bacterially expressed human ORF2


    Predicted band size : 149 kDa

    Note: No specific band staining is seen in the uninduced lane (lane 2). The lower molecular weight bands represent non-specific staining. The band at ~70 kDa (arrowhead) corresponds to a Human L1/ORF2 endonuclease domain fusion protein.

References

ab106004 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab106004.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up