Anti-LINE-1 retrotransposable element ORF2 protein antibody (ab106004)

Overview

  • Product nameAnti-LINE-1 retrotransposable element ORF2 protein antibody
  • Description
    Chicken polyclonal to LINE-1 retrotransposable element ORF2 protein
  • SpecificityThe two synthetic immunogen peptides correspond to regions within the endonuclease domain of L1/ORF2 protein. This antibody is intended for use in studying control of L1 retrotransposons.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human LINE-1 retrotransposable element ORF2 protein conjugated to Keyhole Limpet Haemocyanin (KLH). Two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.
    Database link: O00370

  • Positive control
    • induced bacterially expressed human ORF2
  • General notes

    Dilute only prior to immediate use.
    Expiration date is one (1) year from date of opening product. 

    ab106004 is intended for use in studying control of L1 retrotransposons.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate
  • Concentration information loading...
  • PurityIgY fraction
  • Purification notesab106004 is an IgY fraction antibody purified from monospecific Chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation, followed by extensive dialysis against the buffer.
  • ClonalityPolyclonal
  • IsotypeIgY
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 149 kDa.
ELISA 1/10000.

Anti-LINE-1 retrotransposable element ORF2 protein antibody images

  • All lanes : Anti-LINE-1 retrotransposable element ORF2 protein antibody (ab106004) at 1/2000 dilution

    Lane 1 : induced bacterially expressed human ORF2
    Lane 2 : uninduced bacterially expressed human ORF2


    Predicted band size : 149 kDa

    Note: No specific band staining is seen in the uninduced lane (lane 2). The lower molecular weight bands represent non-specific staining. The band at ~70 kDa (arrowhead) corresponds to a Human L1/ORF2 endonuclease domain fusion protein.

References for Anti-LINE-1 retrotransposable element ORF2 protein antibody (ab106004)

ab106004 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"