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Lipase Activity Assay Kit (Colorimetric) (ab102524) is a product where lipase hydrolyzes a triglyceride substrate to form glycerol which is quantified enzymatically by via monitoring a linked change in the OxiRed probe absorbance (OD=570nm).
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This assay is rapid, simple, sensitive, and reliable for activity screening of lipase. This kit detects lipase activity as low as 0.02mU per well.
For HTP analysis, we recommend Lipase Detection Kit II (Colorimetric) (ab102525) or Lipase Detection Kit III (Fluorometric) (ab118969).
|Assay Buffer||WM||1 x 25ml|
|Enzyme Mix (lyophilized)||Green||1 vial|
|Glycerol Standard||Yellow||1 x 0.2ml|
|Lipase positive control||Purple||1 vial|
|Lipase Substrate||Blue||1 vial|
|OxiRed Probe||Red||1 x 0.2ml|
Our Abpromise guarantee covers the use of ab102524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|