Overview

  • Product name
    Lipase Activity Assay Kit (Colorimetric)
    See all Lipase kits
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
  • Assay type
    Enzyme activity
  • Sensitivity
    0.02 mU/well
  • Assay time
    1h 30m
  • Product overview

    Lipase Activity Assay Kit (Colorimetric) (ab102524) is a product where lipase hydrolyzes a triglyceride substrate to form glycerol which is quantified enzymatically by via monitoring a linked change in the OxiRed probe absorbance (OD=570nm).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This assay is rapid, simple, sensitive, and reliable for activity screening of lipase. This kit detects lipase activity as low as 0.02mU per well.

    For HTP analysis, we recommend Lipase Activity Assay Kit II (Colorimetric) (ab102525) or Lipase Activity Assay Kit III (Fluorometric) (ab118969).

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab102524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Images

  • Sample Timeline
  • Glycerol standard Curve
  • Plasma lipase levels were measured (using ab102524) after 75 days treatment with saline, liraglutide, exendin-4 or sitagliptin. ND, normal chow diet; HFD, high fat diet. p≤0.05, *; p≤0.01, **, n = 3–7 mice.

    There were no detectable differences in plasma lipase activity in mice on a normal chow diet administered any of the three drugs when compared to animals administered saline. Likewise, there was no significant change in plasma lipase activity in mice that were administered saline on a high fat diet vsnormal diet. Furthermore, administration of liraglutide and exendin-4 in combination with a high fat diet also failed to affect plasma lipase activity. We observed no detectable changes in plasma lipase activity in animals maintained on a normal chow diet and administered any of the three drugs when compared to animals administered saline.

Protocols

References

This product has been referenced in:
  • Sakamuri SSVP  et al. Absence of Tissue Inhibitor of Metalloproteinase-4 (TIMP4) ameliorates high fat diet-induced obesity in mice due to defective lipid absorption. Sci Rep 7:6210 (2017). Read more (PubMed: 28740132) »
  • Mondragon A  et al. Divergent effects of liraglutide, exendin-4, and sitagliptin on beta-cell mass and indicators of pancreatitis in a mouse model of hyperglycaemia. PLoS One 9:e104873 (2014). Read more (PubMed: 25119717) »

See all 2 Publications for this product

Customer reviews and Q&As


Since the lab has not tried vegetable oil themselves, they do not have any recommendations/ comments on what the most optimal protocol for such use would be. That would have to be optimized by the end user.

Thank you for your inquiry.

I heard back from the lab that the efficiency of the assay depends on how well the serum was frozen, how many times it has been freeze thawed since then etc.

If the serum is of good quality, the lab sugge...

Read More

the Assay Buffer included with the kit can be used to open cells, thus producing a homogenate that can be assed for lipase activity.

It is recommended to use a dounce homogenizer to homogenize cells or tissues here, although your biomasher ma...

Read More

Thank you for contacting Abcam.

The substrate for ab102524 is also a triglyceride derivative. The datasheet product overview states, "In Abcam's Lipase Assay Kit (Colorimetric), lipase hydrolyzes a triglyceride substrate to form glycerol ...

Read More

Thank you for your patience.

I am sorry this kit component did not conatin the amount as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx. T...

Read More

Thank you for contacting us and for letting us know about this issue.

I am in touch with the supplying lab who has agreed to provide another vial of the glycerol standard as well as with our internal department to arrange for this.

...

Read More

Thank you for your email.

I have received the following email form lab;

For the standards, you have to take the OD only at the final time point. The OD values of the various concentrations of glycerol will increase with time and final...

Read More

Thank you for sending the data.

Unfortunately the graph only, is not enough for the data analysis. We need to check all the protocol details e.g. species, type of samples used, sample dilutions and the raw data etc.

I just had chat w...

Read More

Thank you for letting me know.

I don't know what could have happened there but if you have any similar problems in the future please do let us know.

May I ask, when you called this morning, did you speak to any of our Scientific Sup...

Read More

Thank you for contacting us and sorry for the delay in getting back to you.

I am sorry for the confusion caused by how the protocol has been worded for the Lipase Detection Kit (Colorimetric), ab102524. I can confirm that as the protocol stat...

Read More

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up