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Lipase Detection Kit II (Colorimetric) (ab102525)

Q&A (1)

Overview

  • Product nameLipase Detection Kit II (Colorimetric)See all Lipase kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeEnzyme activity
  • Assay time
    1h 30m
  • Product overview

    Abcam's Lipase Detection Kit II (Colorimetric) provides a simple, sensitive, and reliable assay for rapid analysis of Lipase in samples. In the assay, Lipases hydrolyze a specific substrate to generate a product which reacts with the DTNB probe to generate color (λ=412 nm)..
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    This kit is suitable for high throughput analyses (HTP).

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceLipases perform essential roles in the digestion, transport and processing of dietary lipids (e.g. fats and oils) in living organisms. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglyceride to monoglyceride and free fatty acid. Pancreatic lipase monitoring is also used to help diagnose Crohn's disease, cystic fibrosis and celiac disease. Damage to the pancreas can exhibit a 5-10 fold increase of serum lipase levels within 24 to 48 hours.

    • Applications

    Our Abpromise guarantee covers the use of ab102525 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Lipase Detection Kit II (Colorimetric) images

    • Functional Studies - Lipase Detection Kit II (ab102525)
      Functional Studies - Lipase Detection Kit II (ab102525)
      TNB Standard Curve
    • Functional Studies - Lipase Detection Kit II (ab102525)
      Functional Studies - Lipase Detection Kit II (ab102525)
      Lipase Detection in a Sample and Positive Control

    Protocols

    References for Lipase Detection Kit II (Colorimetric) (ab102525)

    ab102525 has not yet been referenced specifically in any publications.

    Publishing research using ab102525 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab102525

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    Hello,

    I have a question regarding your Lipase Detection Kit II (product code ab102525) and Lipase Detection Kit III (ab118969). In order to determine if these products might be potentially useful for our application, I would need to know more ab...

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    Thank you for contacting Abcam.

    The substates for ab102525 and ab118969 are triglyceride derivatives.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Fr...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"