Overview

  • Product nameAnti-LIPG antibody
    See all LIPG primary antibodies
  • Description
    Rabbit polyclonal to LIPG
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human LIPG.

  • Positive control
    • This antibody gave a positive signal in Human kidney tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab100987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 62 kDa (predicted molecular weight: 57 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionHas phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin.
  • Tissue specificityHigh level of expression in the liver, placenta, lung, thyroid, kidney, testis and in the corpus luteum of the ovary. Expressed also in coronary artery endothelial cells, umbilical vein endothelial cells and in hepatocyte and osteosarcoma cell lines. Not detected in heart, brain and muscle.
  • Sequence similaritiesBelongs to the AB hydrolase superfamily. Lipase family.
    Contains 1 PLAT domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • EDL antibody
    • EL antibody
    • Endothelial cell-derived lipase antibody
    • Endothelial lipase antibody
    • Endothelial lipase precursor antibody
    • Lipase endothelial antibody
    • LIPE_HUMAN antibody
    • LIPG antibody
    • Lipoprotein lipase H antibody
    • PRO719 antibody
    see all

Anti-LIPG antibody images

  • Anti-LIPG antibody (ab100987) at 1/250 dilution + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • ICC/IF image of ab100987 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100987, µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa cells at 5µg/ml.

References for Anti-LIPG antibody (ab100987)

ab100987 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton X100 in PBS
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Sep 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"