Overview

  • Product nameAnti-LIPG antibody
    See all LIPG primary antibodies
  • Description
    Rabbit polyclonal to LIPG
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) (N terminal).

  • Positive control
    • Tested with 293 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab14797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 57 kDa.

Target

  • FunctionHas phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin.
  • Tissue specificityHigh level of expression in the liver, placenta, lung, thyroid, kidney, testis and in the corpus luteum of the ovary. Expressed also in coronary artery endothelial cells, umbilical vein endothelial cells and in hepatocyte and osteosarcoma cell lines. Not detected in heart, brain and muscle.
  • Sequence similaritiesBelongs to the AB hydrolase superfamily. Lipase family.
    Contains 1 PLAT domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • EDL antibody
    • EL antibody
    • Endothelial cell-derived lipase antibody
    • Endothelial lipase antibody
    • Endothelial lipase precursor antibody
    • Lipase endothelial antibody
    • LIPE_HUMAN antibody
    • LIPG antibody
    • Lipoprotein lipase H antibody
    • PRO719 antibody
    see all

Anti-LIPG antibody images

  • Human LIPG detected in transfected 293 lysates, using ab14797.

References for Anti-LIPG antibody (ab14797)

This product has been referenced in:
  • Singaraja RR  et al. The impact of partial and complete loss-of-function mutations in endothelial lipase on high-density lipoprotein levels and functionality in humans. Circ Cardiovasc Genet 6:54-62 (2013). WB ; Human . Read more (PubMed: 23243195) »
  • Kivelä AM  et al. Sulforaphane inhibits endothelial lipase expression through NF-?B in endothelial cells. Atherosclerosis 213:122-8 (2010). WB ; Human . Read more (PubMed: 20688330) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HUVEC)
Specification HUVEC
Fixative Paraformaldehyde
Permeabilization Yes - TritonX100 0.1%
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
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Abcam user community

Verified customer

Submitted Jul 09 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HUVEC)
Loading amount 25 µg
Specification HUVEC
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jun 06 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"