Overview

  • Product nameAnti-LIPG antibody
    See all LIPG primary antibodies
  • Description
    Mouse monoclonal to LIPG
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 21-501 of Human LIPG

Properties

Applications

Our Abpromise guarantee covers the use of ab56493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionHas phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin.
    • Tissue specificityHigh level of expression in the liver, placenta, lung, thyroid, kidney, testis and in the corpus luteum of the ovary. Expressed also in coronary artery endothelial cells, umbilical vein endothelial cells and in hepatocyte and osteosarcoma cell lines. Not detected in heart, brain and muscle.
    • Sequence similaritiesBelongs to the AB hydrolase superfamily. Lipase family.
      Contains 1 PLAT domain.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • EDL antibody
      • EL antibody
      • Endothelial cell-derived lipase antibody
      • Endothelial lipase antibody
      • Endothelial lipase precursor antibody
      • Lipase endothelial antibody
      • LIPE_HUMAN antibody
      • LIPG antibody
      • Lipoprotein lipase H antibody
      • PRO719 antibody
      see all

    Anti-LIPG antibody images

    • Western blot against tagged recombinant protein immunogen using ab56493 LIPG antibody at 1ug/ml. Predicted band size of immunogen is 79 kDa
    • Anti-LIPG antibody (ab56493) at 1/1000 dilution + whole cell lysate prepared from human HUVEC cells at 25 µg

      Secondary
      Rabbit anti-mouse IRDye 800CW at 1/5000 dilution

      Performed under reducing conditions.

      Observed band size : 80 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 minutes

      This image is courtesy of an anonymous abreview.

      See Abreview

    References for Anti-LIPG antibody (ab56493)

    ab56493 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HUVEC)
    Loading amount 25 µg
    Specification HUVEC
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
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    Verified customer

    Submitted Dec 29 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"