The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/16000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 57 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionHas phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin.
Tissue specificityHigh level of expression in the liver, placenta, lung, thyroid, kidney, testis and in the corpus luteum of the ovary. Expressed also in coronary artery endothelial cells, umbilical vein endothelial cells and in hepatocyte and osteosarcoma cell lines. Not detected in heart, brain and muscle.
Sequence similaritiesBelongs to the AB hydrolase superfamily. Lipase family. Contains 1 PLAT domain.