The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/20000. Detects a band of approximately 63 kDa (predicted molecular weight: 51 kDa).
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Hydrolyzes specifically phosphatidic acid (PA) to produce 2-acyl lysophosphatidic acid (LPA; a potent bioactive lipid mediator) and fatty acid. Does not hydrolyze other phospholipids, like phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) or triacylglycerol (TG).
Present in intestine (at protein level). Expressed in colon, prostate, kidney, pancreas, ovary, testis, intestine, lung and pancreas. Expressed at lower level in brain, spleen and heart. In hair, it is prominently expressed in hair follicles, including the stem cell-rich bulge region.
Involvement in disease
Defects in LIPH are the cause of hypotrichosis localized autosomal recessive type 2 (LAH2) [MIM:604379]; also known as alopecia universalis congenita Mari type. A condition characterized by the presence of less than the normal amount of hair. Affected individuals have sparse or absent scalp, axillary and body hair and sparse eyebrows and eyelashes. Defects in LIPH are the cause of woolly hair autosomal recessive type 2 (ARWH2) [MIM:604379]. A hair shaft disorder characterized by fine and tightly curled hair. Compared to normal curly hair that is observed in some populations, woolly hair grows slowly and stops growing after a few inches. Under light microscopy, woolly hair shows some structural anomalies, including trichorrexis nodosa and tapered ends. Some individuals may present with hypotrichosis.
Belongs to the AB hydrolase superfamily. Lipase family.
Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling LIPH with ab192615 at 1/1000 dilution (1μg/ml). A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Western blot - Anti-LIPH antibody [EP12065] (ab192615)
All lanes : Anti-LIPH antibody [EP12065] (ab192615) at 1/5000 dilution
Lane 1 : Human pancreas tissue lysate Lane 2 : Human prostate cancer tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution